Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism

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Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid : evidence for a hydroxyl-radical-like mechanism. / Kennett, Eleanor C; Davies, Michael Jonathan.

In: Free Radical Biology & Medicine, Vol. 42, No. 8, 15.04.2007, p. 1278-89.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kennett, EC & Davies, MJ 2007, 'Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism', Free Radical Biology & Medicine, vol. 42, no. 8, pp. 1278-89. https://doi.org/10.1016/j.freeradbiomed.2007.01.030

APA

Kennett, E. C., & Davies, M. J. (2007). Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism. Free Radical Biology & Medicine, 42(8), 1278-89. https://doi.org/10.1016/j.freeradbiomed.2007.01.030

Vancouver

Kennett EC, Davies MJ. Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism. Free Radical Biology & Medicine. 2007 Apr 15;42(8):1278-89. https://doi.org/10.1016/j.freeradbiomed.2007.01.030

Author

Kennett, Eleanor C ; Davies, Michael Jonathan. / Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid : evidence for a hydroxyl-radical-like mechanism. In: Free Radical Biology & Medicine. 2007 ; Vol. 42, No. 8. pp. 1278-89.

Bibtex

@article{cc4ba19207d0424789107a51bfd13652,
title = "Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism",
abstract = "The oxidant peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) is generated at sites of inflammation via reaction of O2.- with .NO. Previous studies have shown that these species can oxidize cellular targets, but few data are available on damage to extracellular matrix and its components, despite evidence for matrix modification in a number of pathologies. In the current study we show that reaction of ONOO-/ONOOH with glycosaminoglycans results in extensive polymer fragmentation. Bolus authentic ONOO-/ONOOH modifies hyaluronan, heparin, and chondroitin, dermatan, and heparan sulfates, in a concentration-dependent, but O2-independent, manner. The ONOO-/ONOOH generator 3-(4-morpholinyl)sydnoneimine produces similar time- and concentration-dependent damage. These reactions generate specific polymer fragments via cleavage at disaccharide intervals. Studies at different pH values, and in the presence of bicarbonate, are consistent with ONOOH, rather than the carbonate adduct, CO3.- or ONOO-, being the source of damage. EPR spin trapping experiments have provided evidence for the formation of carbon-centered radicals on glycosaminoglycans and related monosaccharides; the similarity of these spectra to those obtained with authentic HO. is consistent with fragmentation being induced by this oxidant. These data suggest that extracellular matrix fragmentation at sites of inflammation may be due, in part, to the formation and reactions of ONOOH.",
keywords = "Chondroitin, Electron Spin Resonance Spectroscopy, Extracellular Matrix, Glycosaminoglycans, Heparin, Hyaluronic Acid, Hydroxyl Radical, Kinetics, Peroxynitrous Acid, Solutions",
author = "Kennett, {Eleanor C} and Davies, {Michael Jonathan}",
year = "2007",
month = "4",
day = "15",
doi = "10.1016/j.freeradbiomed.2007.01.030",
language = "English",
volume = "42",
pages = "1278--89",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid

T2 - evidence for a hydroxyl-radical-like mechanism

AU - Kennett, Eleanor C

AU - Davies, Michael Jonathan

PY - 2007/4/15

Y1 - 2007/4/15

N2 - The oxidant peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) is generated at sites of inflammation via reaction of O2.- with .NO. Previous studies have shown that these species can oxidize cellular targets, but few data are available on damage to extracellular matrix and its components, despite evidence for matrix modification in a number of pathologies. In the current study we show that reaction of ONOO-/ONOOH with glycosaminoglycans results in extensive polymer fragmentation. Bolus authentic ONOO-/ONOOH modifies hyaluronan, heparin, and chondroitin, dermatan, and heparan sulfates, in a concentration-dependent, but O2-independent, manner. The ONOO-/ONOOH generator 3-(4-morpholinyl)sydnoneimine produces similar time- and concentration-dependent damage. These reactions generate specific polymer fragments via cleavage at disaccharide intervals. Studies at different pH values, and in the presence of bicarbonate, are consistent with ONOOH, rather than the carbonate adduct, CO3.- or ONOO-, being the source of damage. EPR spin trapping experiments have provided evidence for the formation of carbon-centered radicals on glycosaminoglycans and related monosaccharides; the similarity of these spectra to those obtained with authentic HO. is consistent with fragmentation being induced by this oxidant. These data suggest that extracellular matrix fragmentation at sites of inflammation may be due, in part, to the formation and reactions of ONOOH.

AB - The oxidant peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) is generated at sites of inflammation via reaction of O2.- with .NO. Previous studies have shown that these species can oxidize cellular targets, but few data are available on damage to extracellular matrix and its components, despite evidence for matrix modification in a number of pathologies. In the current study we show that reaction of ONOO-/ONOOH with glycosaminoglycans results in extensive polymer fragmentation. Bolus authentic ONOO-/ONOOH modifies hyaluronan, heparin, and chondroitin, dermatan, and heparan sulfates, in a concentration-dependent, but O2-independent, manner. The ONOO-/ONOOH generator 3-(4-morpholinyl)sydnoneimine produces similar time- and concentration-dependent damage. These reactions generate specific polymer fragments via cleavage at disaccharide intervals. Studies at different pH values, and in the presence of bicarbonate, are consistent with ONOOH, rather than the carbonate adduct, CO3.- or ONOO-, being the source of damage. EPR spin trapping experiments have provided evidence for the formation of carbon-centered radicals on glycosaminoglycans and related monosaccharides; the similarity of these spectra to those obtained with authentic HO. is consistent with fragmentation being induced by this oxidant. These data suggest that extracellular matrix fragmentation at sites of inflammation may be due, in part, to the formation and reactions of ONOOH.

KW - Chondroitin

KW - Electron Spin Resonance Spectroscopy

KW - Extracellular Matrix

KW - Glycosaminoglycans

KW - Heparin

KW - Hyaluronic Acid

KW - Hydroxyl Radical

KW - Kinetics

KW - Peroxynitrous Acid

KW - Solutions

U2 - 10.1016/j.freeradbiomed.2007.01.030

DO - 10.1016/j.freeradbiomed.2007.01.030

M3 - Journal article

C2 - 17382208

VL - 42

SP - 1278

EP - 1289

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 8

ER -

ID: 129671257