Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid

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Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid. / Kennett, Eleanor C; Davies, Michael Jonathan.

In: Free Radical Biology & Medicine, Vol. 45, No. 5, 01.09.2008, p. 716-25.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kennett, EC & Davies, MJ 2008, 'Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid', Free Radical Biology & Medicine, vol. 45, no. 5, pp. 716-25. https://doi.org/10.1016/j.freeradbiomed.2008.05.027

APA

Kennett, E. C., & Davies, M. J. (2008). Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid. Free Radical Biology & Medicine, 45(5), 716-25. https://doi.org/10.1016/j.freeradbiomed.2008.05.027

Vancouver

Kennett EC, Davies MJ. Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid. Free Radical Biology & Medicine. 2008 Sep 1;45(5):716-25. https://doi.org/10.1016/j.freeradbiomed.2008.05.027

Author

Kennett, Eleanor C ; Davies, Michael Jonathan. / Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid. In: Free Radical Biology & Medicine. 2008 ; Vol. 45, No. 5. pp. 716-25.

Bibtex

@article{dbd71715375b4e15b4723185c794a553,
title = "Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid",
abstract = "The extracellular matrix (ECM) provides strength and elasticity to tissues and plays a key role in regulating cell behavior; damage to this material is believed to be a major factor in many inflammatory diseases. Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; however, the mechanisms involved are poorly understood. Here we examined the reactions of bolus peroxynitrite, and that generated in a time-dependent manner by SIN-1 decomposition, with ECM isolated from a vascular smooth muscle cell line and porcine thoracic aorta. Bolus peroxynitrite caused the release of ECM glycosaminoglycans and proteins, the formation of 3-nitroTyr, and the detection of ECM-derived radicals (by immuno-spin trapping) in a concentration-dependent manner. Release and nitration of ECM components were modulated by the local pH and bicarbonate. SIN-1 caused the release of glycosaminoglycan, but not protein, from vascular smooth muscle cell-derived ECM in a concentration-, time-, and pH-dependent manner. The data presented here suggest that peroxynitrite-mediated damage to ECM occurs via a radical-mediated pathway. These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions.",
keywords = "Animals, Bicarbonates, Cells, Cultured, Extracellular Matrix, Hydrogen-Ion Concentration, Molecular Structure, Molsidomine, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Peroxynitrous Acid, Rats, Swine, Tyrosine",
author = "Kennett, {Eleanor C} and Davies, {Michael Jonathan}",
year = "2008",
month = "9",
day = "1",
doi = "10.1016/j.freeradbiomed.2008.05.027",
language = "English",
volume = "45",
pages = "716--25",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid

AU - Kennett, Eleanor C

AU - Davies, Michael Jonathan

PY - 2008/9/1

Y1 - 2008/9/1

N2 - The extracellular matrix (ECM) provides strength and elasticity to tissues and plays a key role in regulating cell behavior; damage to this material is believed to be a major factor in many inflammatory diseases. Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; however, the mechanisms involved are poorly understood. Here we examined the reactions of bolus peroxynitrite, and that generated in a time-dependent manner by SIN-1 decomposition, with ECM isolated from a vascular smooth muscle cell line and porcine thoracic aorta. Bolus peroxynitrite caused the release of ECM glycosaminoglycans and proteins, the formation of 3-nitroTyr, and the detection of ECM-derived radicals (by immuno-spin trapping) in a concentration-dependent manner. Release and nitration of ECM components were modulated by the local pH and bicarbonate. SIN-1 caused the release of glycosaminoglycan, but not protein, from vascular smooth muscle cell-derived ECM in a concentration-, time-, and pH-dependent manner. The data presented here suggest that peroxynitrite-mediated damage to ECM occurs via a radical-mediated pathway. These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions.

AB - The extracellular matrix (ECM) provides strength and elasticity to tissues and plays a key role in regulating cell behavior; damage to this material is believed to be a major factor in many inflammatory diseases. Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; however, the mechanisms involved are poorly understood. Here we examined the reactions of bolus peroxynitrite, and that generated in a time-dependent manner by SIN-1 decomposition, with ECM isolated from a vascular smooth muscle cell line and porcine thoracic aorta. Bolus peroxynitrite caused the release of ECM glycosaminoglycans and proteins, the formation of 3-nitroTyr, and the detection of ECM-derived radicals (by immuno-spin trapping) in a concentration-dependent manner. Release and nitration of ECM components were modulated by the local pH and bicarbonate. SIN-1 caused the release of glycosaminoglycan, but not protein, from vascular smooth muscle cell-derived ECM in a concentration-, time-, and pH-dependent manner. The data presented here suggest that peroxynitrite-mediated damage to ECM occurs via a radical-mediated pathway. These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions.

KW - Animals

KW - Bicarbonates

KW - Cells, Cultured

KW - Extracellular Matrix

KW - Hydrogen-Ion Concentration

KW - Molecular Structure

KW - Molsidomine

KW - Muscle, Smooth, Vascular

KW - Myocytes, Smooth Muscle

KW - Peroxynitrous Acid

KW - Rats

KW - Swine

KW - Tyrosine

U2 - 10.1016/j.freeradbiomed.2008.05.027

DO - 10.1016/j.freeradbiomed.2008.05.027

M3 - Journal article

C2 - 18582557

VL - 45

SP - 716

EP - 725

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 5

ER -

ID: 129670781