Cytokines cause functional and structural damage to isolated islets of Langerhans

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Cytokines cause functional and structural damage to isolated islets of Langerhans. / Mandrup-Poulsen, T; Bendtzen, K; Bendixen, G; Nerup, J; Nielsen, Jens Høiriis.

In: Allergy, Vol. 40, No. 6, 08.1985, p. 424-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mandrup-Poulsen, T, Bendtzen, K, Bendixen, G, Nerup, J & Nielsen, JH 1985, 'Cytokines cause functional and structural damage to isolated islets of Langerhans', Allergy, vol. 40, no. 6, pp. 424-9.

APA

Mandrup-Poulsen, T., Bendtzen, K., Bendixen, G., Nerup, J., & Nielsen, J. H. (1985). Cytokines cause functional and structural damage to isolated islets of Langerhans. Allergy, 40(6), 424-9.

Vancouver

Mandrup-Poulsen T, Bendtzen K, Bendixen G, Nerup J, Nielsen JH. Cytokines cause functional and structural damage to isolated islets of Langerhans. Allergy. 1985 Aug;40(6):424-9.

Author

Mandrup-Poulsen, T ; Bendtzen, K ; Bendixen, G ; Nerup, J ; Nielsen, Jens Høiriis. / Cytokines cause functional and structural damage to isolated islets of Langerhans. In: Allergy. 1985 ; Vol. 40, No. 6. pp. 424-9.

Bibtex

@article{0a3ecf570b224fa591e55102b8d4292b,
title = "Cytokines cause functional and structural damage to isolated islets of Langerhans",
abstract = "Cytokines are soluble, antigen non-specific, non-immunoglobulin mediators produced and secreted by blood mononuclear cells interacting in the cellular immune-response. To test the possibility that cytokines participate in the autoimmune destruction of the pancreatic beta-cells leading to insulin-dependent diabetes mellitus, isolated human or rat islets of Langerhans were incubated for 7 days with cytokine-rich, cell-free supernatants of blood mononuclear cells from healthy human donors stimulated with or without purified protein derivative of tuberculin or phytohaemagglutinin. Glucose stimulated insulin-release, and contents of insulin and glucagon in islets incubated with cytokine-rich supernatants were markedly reduced. This impairment of islet function was due to a cytotoxic effect of cytokine-rich supernatants as judged by disintegration of normal light-microscopic morphology.",
keywords = "Animals, Biological Agents, Culture Techniques, Cytokines, Cytotoxicity, Immunologic, Glucose, Humans, Immunity, Cellular, Insulin, Islets of Langerhans, Male, Monocytes, Rats, Secretory Rate, Time Factors",
author = "T Mandrup-Poulsen and K Bendtzen and G Bendixen and J Nerup and Nielsen, {Jens H{\o}iriis}",
year = "1985",
month = aug,
language = "English",
volume = "40",
pages = "424--9",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley Online",
number = "6",

}

RIS

TY - JOUR

T1 - Cytokines cause functional and structural damage to isolated islets of Langerhans

AU - Mandrup-Poulsen, T

AU - Bendtzen, K

AU - Bendixen, G

AU - Nerup, J

AU - Nielsen, Jens Høiriis

PY - 1985/8

Y1 - 1985/8

N2 - Cytokines are soluble, antigen non-specific, non-immunoglobulin mediators produced and secreted by blood mononuclear cells interacting in the cellular immune-response. To test the possibility that cytokines participate in the autoimmune destruction of the pancreatic beta-cells leading to insulin-dependent diabetes mellitus, isolated human or rat islets of Langerhans were incubated for 7 days with cytokine-rich, cell-free supernatants of blood mononuclear cells from healthy human donors stimulated with or without purified protein derivative of tuberculin or phytohaemagglutinin. Glucose stimulated insulin-release, and contents of insulin and glucagon in islets incubated with cytokine-rich supernatants were markedly reduced. This impairment of islet function was due to a cytotoxic effect of cytokine-rich supernatants as judged by disintegration of normal light-microscopic morphology.

AB - Cytokines are soluble, antigen non-specific, non-immunoglobulin mediators produced and secreted by blood mononuclear cells interacting in the cellular immune-response. To test the possibility that cytokines participate in the autoimmune destruction of the pancreatic beta-cells leading to insulin-dependent diabetes mellitus, isolated human or rat islets of Langerhans were incubated for 7 days with cytokine-rich, cell-free supernatants of blood mononuclear cells from healthy human donors stimulated with or without purified protein derivative of tuberculin or phytohaemagglutinin. Glucose stimulated insulin-release, and contents of insulin and glucagon in islets incubated with cytokine-rich supernatants were markedly reduced. This impairment of islet function was due to a cytotoxic effect of cytokine-rich supernatants as judged by disintegration of normal light-microscopic morphology.

KW - Animals

KW - Biological Agents

KW - Culture Techniques

KW - Cytokines

KW - Cytotoxicity, Immunologic

KW - Glucose

KW - Humans

KW - Immunity, Cellular

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Monocytes

KW - Rats

KW - Secretory Rate

KW - Time Factors

M3 - Journal article

C2 - 3901813

VL - 40

SP - 424

EP - 429

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 6

ER -

ID: 47975131