Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice

Research output: Contribution to journal › Journal article › Research › peer-review

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Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice. / Christophersen, Daniel Vest; Raun Jacobsen, Nicklas; Guerra Andersen, Maria Helena; Connell, Shea P.; Barfod, Kenneth K.; Thomsen, Morten Bækgaard; Miller, Mark R.; Duffin, Rodger; Lykkesfeldt, Jens; Vogel, Ulla Birgitte; Wallin, Erik Håkan Richard; Loft, Steffen; Roursgaard, Martin; Møller, Peter.

In: Toxicology, Vol. 371, 14.09.2016, p. 29-40.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christophersen, DV, Raun Jacobsen, N, Guerra Andersen, MH, Connell, SP, Barfod, KK, Thomsen, MB, Miller, MR, Duffin, R, Lykkesfeldt, J, Vogel, UB, Wallin, EHR, Loft, S, Roursgaard, M & Møller, P 2016, 'Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice', Toxicology, vol. 371, pp. 29-40. https://doi.org/10.1016/j.tox.2016.10.003

APA

Christophersen, D. V., Raun Jacobsen, N., Guerra Andersen, M. H., Connell, S. P., Barfod, K. K., Thomsen, M. B., Miller, M. R., Duffin, R., Lykkesfeldt, J., Vogel, U. B., Wallin, E. H. R., Loft, S., Roursgaard, M., & Møller, P. (2016). Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice. Toxicology, 371, 29-40. https://doi.org/10.1016/j.tox.2016.10.003

Vancouver

Christophersen DV, Raun Jacobsen N, Guerra Andersen MH, Connell SP, Barfod KK, Thomsen MB et al. Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice. Toxicology. 2016 Sep 14;371:29-40. https://doi.org/10.1016/j.tox.2016.10.003

Author

Christophersen, Daniel Vest ; Raun Jacobsen, Nicklas ; Guerra Andersen, Maria Helena ; Connell, Shea P. ; Barfod, Kenneth K. ; Thomsen, Morten Bækgaard ; Miller, Mark R. ; Duffin, Rodger ; Lykkesfeldt, Jens ; Vogel, Ulla Birgitte ; Wallin, Erik Håkan Richard ; Loft, Steffen ; Roursgaard, Martin ; Møller, Peter. / Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice. In: Toxicology. 2016 ; Vol. 371. pp. 29-40.

Bibtex

@article{20a9f19c292b433d988fe8f4a131b0ae,

title = "Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice",

abstract = "Exposure to high aspect ratio nanomaterials, such as multi-walled carbon nanotubes (MWCNTs) may be associated with increased risk of atherosclerosis, pulmonary disease, and cancer. In the present study, we investigated the cardiovascular and pulmonary health effects of 10 weeks of repeated oral or pulmonary exposures to MWCNTs (4 or 40μg each week) in Apolipoprotein E-deficient (ApoE(-/-)) mice fed a Western-type diet. Intratracheal instillation of MWCNTs was associated with oxidative damage to DNA in lung tissue and elevated levels of lipid peroxidation products in plasma, whereas the exposure only caused a modest pulmonary inflammation in terms of increased numbers of lymphocytes in bronchoalveolar lavage fluid. Ultrasound imaging in live animals revealed an increase in the inner and outer wall thickness of the aortic arch at 10 weeks after pulmonary exposure to MWCNTs, which may suggest artery remodelling. However, we did not find accelerated plaque progression in the aorta or the brachiocephalic artery by histopathology. Furthermore, repeated oral exposure to MWCNTs did not cause changes in the composition of gut microbiota of exposed mice. Collectively, this study indicates that repeated pulmonary exposure to MWCNTs was associated with oxidative stress, whereas cardiovascular effects encompassed remodelling of the aorta wall.",

author = "Christophersen, {Daniel Vest} and {Raun Jacobsen}, Nicklas and {Guerra Andersen}, {Maria Helena} and Connell, {Shea P.} and Barfod, {Kenneth K.} and Thomsen, {Morten B{\ae}kgaard} and Miller, {Mark R.} and Rodger Duffin and Jens Lykkesfeldt and Vogel, {Ulla Birgitte} and Wallin, {Erik H{\aa}kan Richard} and Steffen Loft and Martin Roursgaard and Peter M{\o}ller",

note = "Copyright {\textcopyright} 2016. Published by Elsevier Ireland Ltd.",

year = "2016",

month = sep,

day = "14",

doi = "10.1016/j.tox.2016.10.003",

language = "English",

volume = "371",

pages = "29--40",

journal = "Toxicology",

issn = "0300-483X",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice

AU - Christophersen, Daniel Vest

AU - Raun Jacobsen, Nicklas

AU - Guerra Andersen, Maria Helena

AU - Connell, Shea P.

AU - Barfod, Kenneth K.

AU - Thomsen, Morten Bækgaard

AU - Miller, Mark R.

AU - Duffin, Rodger

AU - Lykkesfeldt, Jens

AU - Vogel, Ulla Birgitte

AU - Wallin, Erik Håkan Richard

AU - Loft, Steffen

AU - Roursgaard, Martin

AU - Møller, Peter

PY - 2016/9/14

Y1 - 2016/9/14

N2 - Exposure to high aspect ratio nanomaterials, such as multi-walled carbon nanotubes (MWCNTs) may be associated with increased risk of atherosclerosis, pulmonary disease, and cancer. In the present study, we investigated the cardiovascular and pulmonary health effects of 10 weeks of repeated oral or pulmonary exposures to MWCNTs (4 or 40μg each week) in Apolipoprotein E-deficient (ApoE(-/-)) mice fed a Western-type diet. Intratracheal instillation of MWCNTs was associated with oxidative damage to DNA in lung tissue and elevated levels of lipid peroxidation products in plasma, whereas the exposure only caused a modest pulmonary inflammation in terms of increased numbers of lymphocytes in bronchoalveolar lavage fluid. Ultrasound imaging in live animals revealed an increase in the inner and outer wall thickness of the aortic arch at 10 weeks after pulmonary exposure to MWCNTs, which may suggest artery remodelling. However, we did not find accelerated plaque progression in the aorta or the brachiocephalic artery by histopathology. Furthermore, repeated oral exposure to MWCNTs did not cause changes in the composition of gut microbiota of exposed mice. Collectively, this study indicates that repeated pulmonary exposure to MWCNTs was associated with oxidative stress, whereas cardiovascular effects encompassed remodelling of the aorta wall.

AB - Exposure to high aspect ratio nanomaterials, such as multi-walled carbon nanotubes (MWCNTs) may be associated with increased risk of atherosclerosis, pulmonary disease, and cancer. In the present study, we investigated the cardiovascular and pulmonary health effects of 10 weeks of repeated oral or pulmonary exposures to MWCNTs (4 or 40μg each week) in Apolipoprotein E-deficient (ApoE(-/-)) mice fed a Western-type diet. Intratracheal instillation of MWCNTs was associated with oxidative damage to DNA in lung tissue and elevated levels of lipid peroxidation products in plasma, whereas the exposure only caused a modest pulmonary inflammation in terms of increased numbers of lymphocytes in bronchoalveolar lavage fluid. Ultrasound imaging in live animals revealed an increase in the inner and outer wall thickness of the aortic arch at 10 weeks after pulmonary exposure to MWCNTs, which may suggest artery remodelling. However, we did not find accelerated plaque progression in the aorta or the brachiocephalic artery by histopathology. Furthermore, repeated oral exposure to MWCNTs did not cause changes in the composition of gut microbiota of exposed mice. Collectively, this study indicates that repeated pulmonary exposure to MWCNTs was associated with oxidative stress, whereas cardiovascular effects encompassed remodelling of the aorta wall.

U2 - 10.1016/j.tox.2016.10.003

DO - 10.1016/j.tox.2016.10.003

M3 - Journal article

C2 - 27725195

VL - 371

SP - 29

EP - 40

JO - Toxicology

JF - Toxicology

SN - 0300-483X

ER -

ID: 167473920

Department of Biomedical Sciences