During recent years small conductance Ca activated K (SK) channels have been reported to play a role in cardiac electrophysiology. SK channels seem to be expressed in atria and ventricles but from a functional perspective atrial activity is predominant. A general notion seems to be that cardiac SK channels are predominantly coming into play during arrhythmogenic events where intracellular concentration of Ca is increased. During ventricular fibrillation a surge of [Ca]i has the potential to bind to and open SK channels. To obtain mechanistic insight into possible roles of SK channels during ventricular fibrillation we conducted experiments with a SK channel pore blocker (ICA) and a negatively allosteric modulator (NS8395) in a Langendorff perfused heart model. Both compounds increased the action potential duration (APD), effective refractory period (ERP) and Wenckebach cycle length (WCL) to comparable extents. Despite these similarities, the SK channel modulator was found to revert and prevent ventricular fibrillation (VF) more efficiently than the SK channel pore blocker. In conclusion, either negative allosteric modulation of the SK channel with NS8593 is more favorable than pure channel block with ICA or the two compounds have different selectivity profiles which makes NS8593 more antiarrhythmic than ICA in a setting of VF.