TY - JOUR

T1 - Anthocyanin complex niosome gel accelerates oral wound healing

T2 - In vitro and clinical studies

AU - Damrongrungruang, Teerasak

AU - Paphangkorakit, Jarin

AU - Limsitthichaikoon, Sucharat

AU - Khampaenjiraroch, Bhattaranitch

AU - Davies, Michael Jonathan

AU - Sungthong, Bunleu

AU - Priprem, Aroonsri

N1 - Publisher Copyright: © 2021 Elsevier Inc.

PY - 2021

Y1 - 2021

N2 - An anthocyanin complex (AC), composed of extracts of purple waxy corn and blue butterfly pea petals, and AC niosomes, bilayered vesicles of non-ionic surfactants, were compared in in vitro and clinical studies. Cultured fibroblasts subjected to a scratch wound were monitored for cell viability, cell migration, nuclear morphology and protein expression. Scratched cells showed accelerated wound healing activity, returning to normal 24 h after treatment with AC niosomes (0.002 mg/mL). Western blots and immunocytochemistry indicated upregulation of type I, III and IV collagens, fibronectin and laminins in AC niosome-treated scratched cells. A randomized block placebo-controlled double-blind clinical trial in 60 volunteers (18-60 years old) with oral wounds indicated that AC niosome gel accelerated wound closure, reduced pain due to the oral wounds and improved participants' quality of life more than AC gel, triamcinolone gel and placebo gel. These data are consistent with enhanced delivery of AC to fibroblasts by use of niosomes. AC niosomes activated fibroblasts within wounded regions and accelerated wound healing, indicating that AC niosomes have therapeutic potential.

AB - An anthocyanin complex (AC), composed of extracts of purple waxy corn and blue butterfly pea petals, and AC niosomes, bilayered vesicles of non-ionic surfactants, were compared in in vitro and clinical studies. Cultured fibroblasts subjected to a scratch wound were monitored for cell viability, cell migration, nuclear morphology and protein expression. Scratched cells showed accelerated wound healing activity, returning to normal 24 h after treatment with AC niosomes (0.002 mg/mL). Western blots and immunocytochemistry indicated upregulation of type I, III and IV collagens, fibronectin and laminins in AC niosome-treated scratched cells. A randomized block placebo-controlled double-blind clinical trial in 60 volunteers (18-60 years old) with oral wounds indicated that AC niosome gel accelerated wound closure, reduced pain due to the oral wounds and improved participants' quality of life more than AC gel, triamcinolone gel and placebo gel. These data are consistent with enhanced delivery of AC to fibroblasts by use of niosomes. AC niosomes activated fibroblasts within wounded regions and accelerated wound healing, indicating that AC niosomes have therapeutic potential.

KW - Anthocyanin complex

KW - Fibroblasts

KW - Niosome gel

KW - Placebo-controlled trial

KW - Wound healing

U2 - 10.1016/j.nano.2021.102423

DO - 10.1016/j.nano.2021.102423

M3 - Journal article

C2 - 34214683

AN - SCOPUS:85111315551

VL - 37

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

SN - 1549-9634

M1 - 102423

ER -