Altered mitochondrial regulation in quadriceps muscles of patients with COPD

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Altered mitochondrial regulation in quadriceps muscles of patients with COPD. / Naimi, Ashley I; Bourbeau, Jean; Perrault, Helene; Baril, Jacinthe; Wright-Paradis, Cynthia; Rossi, Amanda; Taivassalo, Tanja; Sheel, A William; Rabøl, Rasmus; Dela, Flemming; Boushel, Robert Christopher.

In: Clinical Physiology and Functional Imaging, Vol. 31, No. 2, 2011, p. 124-31.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Naimi, AI, Bourbeau, J, Perrault, H, Baril, J, Wright-Paradis, C, Rossi, A, Taivassalo, T, Sheel, AW, Rabøl, R, Dela, F & Boushel, RC 2011, 'Altered mitochondrial regulation in quadriceps muscles of patients with COPD', Clinical Physiology and Functional Imaging, vol. 31, no. 2, pp. 124-31. https://doi.org/10.1111/j.1475-097X.2010.00988.x

APA

Naimi, A. I., Bourbeau, J., Perrault, H., Baril, J., Wright-Paradis, C., Rossi, A., Taivassalo, T., Sheel, A. W., Rabøl, R., Dela, F., & Boushel, R. C. (2011). Altered mitochondrial regulation in quadriceps muscles of patients with COPD. Clinical Physiology and Functional Imaging, 31(2), 124-31. https://doi.org/10.1111/j.1475-097X.2010.00988.x

Vancouver

Naimi AI, Bourbeau J, Perrault H, Baril J, Wright-Paradis C, Rossi A et al. Altered mitochondrial regulation in quadriceps muscles of patients with COPD. Clinical Physiology and Functional Imaging. 2011;31(2):124-31. https://doi.org/10.1111/j.1475-097X.2010.00988.x

Author

Naimi, Ashley I ; Bourbeau, Jean ; Perrault, Helene ; Baril, Jacinthe ; Wright-Paradis, Cynthia ; Rossi, Amanda ; Taivassalo, Tanja ; Sheel, A William ; Rabøl, Rasmus ; Dela, Flemming ; Boushel, Robert Christopher. / Altered mitochondrial regulation in quadriceps muscles of patients with COPD. In: Clinical Physiology and Functional Imaging. 2011 ; Vol. 31, No. 2. pp. 124-31.

Bibtex

@article{0622424100634680bf919a1f6129cb02,
title = "Altered mitochondrial regulation in quadriceps muscles of patients with COPD",
abstract = "Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s(-1) mg tissue(-1) ), but O(2) flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s(-1) mg tissue(-1) ); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.",
keywords = "Adenosine Diphosphate, Aged, Biopsy, Case-Control Studies, Cell Respiration, Denmark, Electron Transport Chain Complex Proteins, Electron Transport Complex IV, Energy Metabolism, Humans, Lung, Male, Middle Aged, Mitochondria, Muscle, Oxygen Consumption, Pulmonary Disease, Chronic Obstructive, Quadriceps Muscle, Respiratory Function Tests, Succinic Acid",
author = "Naimi, {Ashley I} and Jean Bourbeau and Helene Perrault and Jacinthe Baril and Cynthia Wright-Paradis and Amanda Rossi and Tanja Taivassalo and Sheel, {A William} and Rasmus Rab{\o}l and Flemming Dela and Boushel, {Robert Christopher}",
note = "{\textcopyright} 2010 The Authors. Clinical Physiology and Functional Imaging {\textcopyright} 2010 Scandinavian Society of Clinical Physiology and Nuclear Medicine.",
year = "2011",
doi = "10.1111/j.1475-097X.2010.00988.x",
language = "English",
volume = "31",
pages = "124--31",
journal = "Clinical Physiology and Functional Imaging",
issn = "1475-0961",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Altered mitochondrial regulation in quadriceps muscles of patients with COPD

AU - Naimi, Ashley I

AU - Bourbeau, Jean

AU - Perrault, Helene

AU - Baril, Jacinthe

AU - Wright-Paradis, Cynthia

AU - Rossi, Amanda

AU - Taivassalo, Tanja

AU - Sheel, A William

AU - Rabøl, Rasmus

AU - Dela, Flemming

AU - Boushel, Robert Christopher

N1 - © 2010 The Authors. Clinical Physiology and Functional Imaging © 2010 Scandinavian Society of Clinical Physiology and Nuclear Medicine.

PY - 2011

Y1 - 2011

N2 - Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s(-1) mg tissue(-1) ), but O(2) flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s(-1) mg tissue(-1) ); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.

AB - Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s(-1) mg tissue(-1) ), but O(2) flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s(-1) mg tissue(-1) ); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.

KW - Adenosine Diphosphate

KW - Aged

KW - Biopsy

KW - Case-Control Studies

KW - Cell Respiration

KW - Denmark

KW - Electron Transport Chain Complex Proteins

KW - Electron Transport Complex IV

KW - Energy Metabolism

KW - Humans

KW - Lung

KW - Male

KW - Middle Aged

KW - Mitochondria, Muscle

KW - Oxygen Consumption

KW - Pulmonary Disease, Chronic Obstructive

KW - Quadriceps Muscle

KW - Respiratory Function Tests

KW - Succinic Acid

U2 - 10.1111/j.1475-097X.2010.00988.x

DO - 10.1111/j.1475-097X.2010.00988.x

M3 - Journal article

C2 - 21091605

VL - 31

SP - 124

EP - 131

JO - Clinical Physiology and Functional Imaging

JF - Clinical Physiology and Functional Imaging

SN - 1475-0961

IS - 2

ER -

ID: 33815758