[68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

[68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure. / Bentsen, Simon; Jensen, Jacob Kildevang; Christensen, Esben; Petersen, Lars Ringgaard; Grandjean, Constance Eline; Follin, Bjarke; Madsen, Johanne Straarup; Christensen, Camilla; Clemmensen, Andreas; Binderup, Tina; Hasbak, Philip; Ripa, Rasmus Sejersten; Kjaer, Andreas.

In: Journal of Nuclear Cardiology, Vol. 30, No. 5, 2023, p. 2073 - 2084.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bentsen, S, Jensen, JK, Christensen, E, Petersen, LR, Grandjean, CE, Follin, B, Madsen, JS, Christensen, C, Clemmensen, A, Binderup, T, Hasbak, P, Ripa, RS & Kjaer, A 2023, '[68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure', Journal of Nuclear Cardiology, vol. 30, no. 5, pp. 2073 - 2084. https://doi.org/10.1007/s12350-023-03265-9

APA

Bentsen, S., Jensen, J. K., Christensen, E., Petersen, L. R., Grandjean, C. E., Follin, B., Madsen, J. S., Christensen, C., Clemmensen, A., Binderup, T., Hasbak, P., Ripa, R. S., & Kjaer, A. (2023). [68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure. Journal of Nuclear Cardiology, 30(5), 2073 - 2084. https://doi.org/10.1007/s12350-023-03265-9

Vancouver

Bentsen S, Jensen JK, Christensen E, Petersen LR, Grandjean CE, Follin B et al. [68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure. Journal of Nuclear Cardiology. 2023;30(5):2073 - 2084. https://doi.org/10.1007/s12350-023-03265-9

Author

Bentsen, Simon ; Jensen, Jacob Kildevang ; Christensen, Esben ; Petersen, Lars Ringgaard ; Grandjean, Constance Eline ; Follin, Bjarke ; Madsen, Johanne Straarup ; Christensen, Camilla ; Clemmensen, Andreas ; Binderup, Tina ; Hasbak, Philip ; Ripa, Rasmus Sejersten ; Kjaer, Andreas. / [68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure. In: Journal of Nuclear Cardiology. 2023 ; Vol. 30, No. 5. pp. 2073 - 2084.

Bibtex

@article{1ab83054afdd4b5e8d291d41644a601e,
title = "[68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure",
abstract = "Background: Angiogenesis has increasingly been a target for imaging and treatment over the last decade. The integrin αvβ3 is highly expressed in cells during angiogenesis and are therefore a promising target for imaging. In this study, we aimed to investigate the PET tracer [68Ga]Ga-RGD as a marker of angiogenesis following MI and its ability to predict cardiac functional parameters. Methods: First, the real-time interaction between [68Ga]Ga-RGD and integrin αvβ3 was investigated using surface plasmon resonance (SPR). Second, an animal study was performed to investigate the [68Ga]Ga-RGD uptake in the infarcted area after one and four weeks following MI in a rat model (MI = 68, sham surgery = 36). Finally, the specificity of the [68Ga]Ga-RGD tracer was evaluated ex vivo using histology, autoradiography, gamma counting and flow cytometry. Results: SPR showed that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, forming a strong and stable binding. PET/CT showed a significantly higher uptake of [68Ga]Ga-RGD in the infarcted area compared to sham one week (p < 0.001) and four weeks (p < 0.001) after MI. The uptake of [68Ga]Ga-RGD after one week correlated to end diastolic volume (r = 0.74, p < 0.001) and ejection fraction (r = − 0.71, p < 0.001) after four weeks. Conclusion: This study demonstrates that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, which enables the evaluation of angiogenesis and remodeling. The [68Ga]Ga-RGD uptake after one week indicates that [68Ga]Ga-RGD may be used as an early predictor of cardiac functional parameters and possible development of heart failure after MI. These encouraging data supports the clinical translation and future use in MI patients.",
keywords = "coronary artery disease, Myocardial biology, myocardial ischemia and infarction, positron emission tomography",
author = "Simon Bentsen and Jensen, {Jacob Kildevang} and Esben Christensen and Petersen, {Lars Ringgaard} and Grandjean, {Constance Eline} and Bjarke Follin and Madsen, {Johanne Straarup} and Camilla Christensen and Andreas Clemmensen and Tina Binderup and Philip Hasbak and Ripa, {Rasmus Sejersten} and Andreas Kjaer",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
doi = "10.1007/s12350-023-03265-9",
language = "English",
volume = "30",
pages = "2073 -- 2084",
journal = "Journal of Nuclear Cardiology",
issn = "1071-3581",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - [68Ga]Ga-NODAGA-E[(cRGDyK)]2 angiogenesis PET following myocardial infarction in an experimental rat model predicts cardiac functional parameters and development of heart failure

AU - Bentsen, Simon

AU - Jensen, Jacob Kildevang

AU - Christensen, Esben

AU - Petersen, Lars Ringgaard

AU - Grandjean, Constance Eline

AU - Follin, Bjarke

AU - Madsen, Johanne Straarup

AU - Christensen, Camilla

AU - Clemmensen, Andreas

AU - Binderup, Tina

AU - Hasbak, Philip

AU - Ripa, Rasmus Sejersten

AU - Kjaer, Andreas

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Background: Angiogenesis has increasingly been a target for imaging and treatment over the last decade. The integrin αvβ3 is highly expressed in cells during angiogenesis and are therefore a promising target for imaging. In this study, we aimed to investigate the PET tracer [68Ga]Ga-RGD as a marker of angiogenesis following MI and its ability to predict cardiac functional parameters. Methods: First, the real-time interaction between [68Ga]Ga-RGD and integrin αvβ3 was investigated using surface plasmon resonance (SPR). Second, an animal study was performed to investigate the [68Ga]Ga-RGD uptake in the infarcted area after one and four weeks following MI in a rat model (MI = 68, sham surgery = 36). Finally, the specificity of the [68Ga]Ga-RGD tracer was evaluated ex vivo using histology, autoradiography, gamma counting and flow cytometry. Results: SPR showed that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, forming a strong and stable binding. PET/CT showed a significantly higher uptake of [68Ga]Ga-RGD in the infarcted area compared to sham one week (p < 0.001) and four weeks (p < 0.001) after MI. The uptake of [68Ga]Ga-RGD after one week correlated to end diastolic volume (r = 0.74, p < 0.001) and ejection fraction (r = − 0.71, p < 0.001) after four weeks. Conclusion: This study demonstrates that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, which enables the evaluation of angiogenesis and remodeling. The [68Ga]Ga-RGD uptake after one week indicates that [68Ga]Ga-RGD may be used as an early predictor of cardiac functional parameters and possible development of heart failure after MI. These encouraging data supports the clinical translation and future use in MI patients.

AB - Background: Angiogenesis has increasingly been a target for imaging and treatment over the last decade. The integrin αvβ3 is highly expressed in cells during angiogenesis and are therefore a promising target for imaging. In this study, we aimed to investigate the PET tracer [68Ga]Ga-RGD as a marker of angiogenesis following MI and its ability to predict cardiac functional parameters. Methods: First, the real-time interaction between [68Ga]Ga-RGD and integrin αvβ3 was investigated using surface plasmon resonance (SPR). Second, an animal study was performed to investigate the [68Ga]Ga-RGD uptake in the infarcted area after one and four weeks following MI in a rat model (MI = 68, sham surgery = 36). Finally, the specificity of the [68Ga]Ga-RGD tracer was evaluated ex vivo using histology, autoradiography, gamma counting and flow cytometry. Results: SPR showed that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, forming a strong and stable binding. PET/CT showed a significantly higher uptake of [68Ga]Ga-RGD in the infarcted area compared to sham one week (p < 0.001) and four weeks (p < 0.001) after MI. The uptake of [68Ga]Ga-RGD after one week correlated to end diastolic volume (r = 0.74, p < 0.001) and ejection fraction (r = − 0.71, p < 0.001) after four weeks. Conclusion: This study demonstrates that [68Ga]Ga-RGD has a high affinity for integrin αvβ3, which enables the evaluation of angiogenesis and remodeling. The [68Ga]Ga-RGD uptake after one week indicates that [68Ga]Ga-RGD may be used as an early predictor of cardiac functional parameters and possible development of heart failure after MI. These encouraging data supports the clinical translation and future use in MI patients.

KW - coronary artery disease

KW - Myocardial biology

KW - myocardial ischemia and infarction

KW - positron emission tomography

U2 - 10.1007/s12350-023-03265-9

DO - 10.1007/s12350-023-03265-9

M3 - Journal article

C2 - 37127725

AN - SCOPUS:85154557569

VL - 30

SP - 2073

EP - 2084

JO - Journal of Nuclear Cardiology

JF - Journal of Nuclear Cardiology

SN - 1071-3581

IS - 5

ER -

ID: 347793355