5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels.

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5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels. / Grunnet, Morten; Jespersen, Thomas; Perrier, Jean-François.

In: Journal of Neuroscience Research, Vol. 78, No. 6, 2004, p. 845-54.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Grunnet, M, Jespersen, T & Perrier, J-F 2004, '5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels.', Journal of Neuroscience Research, vol. 78, no. 6, pp. 845-54. https://doi.org/10.1002/jnr.20318

APA

Grunnet, M., Jespersen, T., & Perrier, J-F. (2004). 5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels. Journal of Neuroscience Research, 78(6), 845-54. https://doi.org/10.1002/jnr.20318

Vancouver

Grunnet M, Jespersen T, Perrier J-F. 5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels. Journal of Neuroscience Research. 2004;78(6):845-54. https://doi.org/10.1002/jnr.20318

Author

Grunnet, Morten ; Jespersen, Thomas ; Perrier, Jean-François. / 5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels. In: Journal of Neuroscience Research. 2004 ; Vol. 78, No. 6. pp. 845-54.

Bibtex

@article{1bd00390ab5511ddb5e9000ea68e967b,
title = "5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels.",
abstract = "Small-conductance calcium-activated potassium channels (SK) are responsible for the medium afterhyperpolarisation (mAHP) following action potentials in neurons. Here we tested the ability of serotonin (5-HT) to modulate the activity of SK channels by coexpressing 5-HT1A receptors with different subtypes of SK channels (SK1, SK2, and SK3) in Xenopus laevis oocytes. SK channels were activated by intracellular injection of Cd2+. Subsequent activation of 5-HT1A receptors by 8-OH-DPAT always produced an inhibition of the SK current, showing the existence of a specific pathway between the receptor and the ion channel. To investigate the physiological relevance of this pathway, we characterized the mAHP present after action potentials in spinal motoneurons recorded in a slice preparation from the lumbar spinal cord of the adult turtle. By performing current and voltage clamp recordings, we showed that 8-OH-DPAT specifically inhibited the fraction of the AHP mediated by SK channels. We conclude that the activity of SK channels is modulated by activation of serotonergic receptors.",
author = "Morten Grunnet and Thomas Jespersen and Jean-Fran{\c c}ois Perrier",
note = "Keywords: 8-Hydroxy-2-(di-n-propylamino)tetralin; Animals; Apamin; Cloning, Molecular; Female; Humans; Oocytes; Potassium Channels, Calcium-Activated; Rats; Receptor, Serotonin, 5-HT1A; Recombinant Proteins; Xenopus laevis",
year = "2004",
doi = "10.1002/jnr.20318",
language = "English",
volume = "78",
pages = "845--54",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "JohnWiley & Sons, Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - 5-HT1A receptors modulate small-conductance Ca2+-activated K+ channels.

AU - Grunnet, Morten

AU - Jespersen, Thomas

AU - Perrier, Jean-François

N1 - Keywords: 8-Hydroxy-2-(di-n-propylamino)tetralin; Animals; Apamin; Cloning, Molecular; Female; Humans; Oocytes; Potassium Channels, Calcium-Activated; Rats; Receptor, Serotonin, 5-HT1A; Recombinant Proteins; Xenopus laevis

PY - 2004

Y1 - 2004

N2 - Small-conductance calcium-activated potassium channels (SK) are responsible for the medium afterhyperpolarisation (mAHP) following action potentials in neurons. Here we tested the ability of serotonin (5-HT) to modulate the activity of SK channels by coexpressing 5-HT1A receptors with different subtypes of SK channels (SK1, SK2, and SK3) in Xenopus laevis oocytes. SK channels were activated by intracellular injection of Cd2+. Subsequent activation of 5-HT1A receptors by 8-OH-DPAT always produced an inhibition of the SK current, showing the existence of a specific pathway between the receptor and the ion channel. To investigate the physiological relevance of this pathway, we characterized the mAHP present after action potentials in spinal motoneurons recorded in a slice preparation from the lumbar spinal cord of the adult turtle. By performing current and voltage clamp recordings, we showed that 8-OH-DPAT specifically inhibited the fraction of the AHP mediated by SK channels. We conclude that the activity of SK channels is modulated by activation of serotonergic receptors.

AB - Small-conductance calcium-activated potassium channels (SK) are responsible for the medium afterhyperpolarisation (mAHP) following action potentials in neurons. Here we tested the ability of serotonin (5-HT) to modulate the activity of SK channels by coexpressing 5-HT1A receptors with different subtypes of SK channels (SK1, SK2, and SK3) in Xenopus laevis oocytes. SK channels were activated by intracellular injection of Cd2+. Subsequent activation of 5-HT1A receptors by 8-OH-DPAT always produced an inhibition of the SK current, showing the existence of a specific pathway between the receptor and the ion channel. To investigate the physiological relevance of this pathway, we characterized the mAHP present after action potentials in spinal motoneurons recorded in a slice preparation from the lumbar spinal cord of the adult turtle. By performing current and voltage clamp recordings, we showed that 8-OH-DPAT specifically inhibited the fraction of the AHP mediated by SK channels. We conclude that the activity of SK channels is modulated by activation of serotonergic receptors.

U2 - 10.1002/jnr.20318

DO - 10.1002/jnr.20318

M3 - Journal article

C2 - 15521063

VL - 78

SP - 845

EP - 854

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 6

ER -

ID: 8418823