Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup. / Steffen, Jonas Hyld; Missel, Julie Winkel; Al-Jubair, Tamim; Kitchen, Philip; Salman, Mootaz M.; Bill, Roslyn M.; Törnroth-Horsefield, Susanna; Gourdon, Pontus.
I: STAR Protocols, Bind 3, Nr. 2, 101312, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup
AU - Steffen, Jonas Hyld
AU - Missel, Julie Winkel
AU - Al-Jubair, Tamim
AU - Kitchen, Philip
AU - Salman, Mootaz M.
AU - Bill, Roslyn M.
AU - Törnroth-Horsefield, Susanna
AU - Gourdon, Pontus
N1 - Publisher Copyright: © 2022 The Authors
PY - 2022
Y1 - 2022
N2 - Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).
AB - Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).
KW - Cell Membrane
KW - Protein Biochemistry
KW - Single-molecule Assays
UR - http://www.scopus.com/inward/record.url?scp=85128333189&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2022.101312
DO - 10.1016/j.xpro.2022.101312
M3 - Journal article
C2 - 35496800
AN - SCOPUS:85128333189
VL - 3
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 2
M1 - 101312
ER -
ID: 311124113