Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)
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We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.
Original language | English |
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Journal | FEBS Letters |
Volume | 229 |
Issue number | 1 |
Pages (from-to) | 175-8 |
Number of pages | 4 |
ISSN | 0014-5793 |
Publication status | Published - 29 Feb 1988 |
- Adenoma, Islet Cell, Animals, Binding, Competitive, Glucagon, Glucagon-Like Peptide 1, Insulin, Insulinoma, Intestines, Kinetics, Pancreatic Neoplasms, Peptides, Rats, Receptors, Cell Surface, Receptors, Glucagon
Research areas
ID: 47974722