Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro

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Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro. / Billestrup, Nils; Swanson, L W; Vale, W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 18, 09.1986, p. 6854-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Billestrup, N, Swanson, LW & Vale, W 1986, 'Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro', Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 18, pp. 6854-7.

APA

Billestrup, N., Swanson, L. W., & Vale, W. (1986). Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro. Proceedings of the National Academy of Sciences of the United States of America, 83(18), 6854-7.

Vancouver

Billestrup N, Swanson LW, Vale W. Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro. Proceedings of the National Academy of Sciences of the United States of America. 1986 Sep;83(18):6854-7.

Author

Billestrup, Nils ; Swanson, L W ; Vale, W. / Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 18. pp. 6854-7.

Bibtex

@article{7814ea7771c04e5d95943b94e2b8c74e,
title = "Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro",
abstract = "The mitogenic effect of the hypothalamic peptides growth hormone-releasing factor (GRF) and somatostatin on cultured growth hormone (GH)-producing cells (somatotrophs) was studied. Using autoradiographic detection of [3H]thymidine uptake and immunocytochemical identification of GH-producing cells, we show that 5 nM GRF causes a 20-fold increase in the percentage of somatotrophs labeled with [3H]thymidine. The total number of somatotrophs in GRF-treated cultures was increased by 60%. Somatostatin had no measurable effect on the labeling index by itself, but it partly inhibited the GRF-induced increase in both the labeling index and the total number of cells. Forskolin caused an increase in both the percentage of somatotrophs with a [3H]thymidine-labeled nucleus and the somatotroph number similar to that caused by GRF. GH secretion as well as cellular GH content in the GRF- or forskolin-treated cells increased with culture time over the entire period, whereas secretion and content of GH gradually decreased in control or somatostatin-treated cultures during the entire culture period. These data suggest that GRF and somatostatin regulate the mitotic activity of GH-producing cells and that the effect of GRF is possibly mediated by cyclic AMP.",
keywords = "Animals, Cell Division, Cells, Cultured, Cyclic AMP, Growth Hormone, Growth Hormone-Releasing Hormone, Male, Pituitary Gland, Anterior, Rats, Rats, Inbred Strains",
author = "Nils Billestrup and Swanson, {L W} and W Vale",
year = "1986",
month = sep,
language = "English",
volume = "83",
pages = "6854--7",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "18",

}

RIS

TY - JOUR

T1 - Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro

AU - Billestrup, Nils

AU - Swanson, L W

AU - Vale, W

PY - 1986/9

Y1 - 1986/9

N2 - The mitogenic effect of the hypothalamic peptides growth hormone-releasing factor (GRF) and somatostatin on cultured growth hormone (GH)-producing cells (somatotrophs) was studied. Using autoradiographic detection of [3H]thymidine uptake and immunocytochemical identification of GH-producing cells, we show that 5 nM GRF causes a 20-fold increase in the percentage of somatotrophs labeled with [3H]thymidine. The total number of somatotrophs in GRF-treated cultures was increased by 60%. Somatostatin had no measurable effect on the labeling index by itself, but it partly inhibited the GRF-induced increase in both the labeling index and the total number of cells. Forskolin caused an increase in both the percentage of somatotrophs with a [3H]thymidine-labeled nucleus and the somatotroph number similar to that caused by GRF. GH secretion as well as cellular GH content in the GRF- or forskolin-treated cells increased with culture time over the entire period, whereas secretion and content of GH gradually decreased in control or somatostatin-treated cultures during the entire culture period. These data suggest that GRF and somatostatin regulate the mitotic activity of GH-producing cells and that the effect of GRF is possibly mediated by cyclic AMP.

AB - The mitogenic effect of the hypothalamic peptides growth hormone-releasing factor (GRF) and somatostatin on cultured growth hormone (GH)-producing cells (somatotrophs) was studied. Using autoradiographic detection of [3H]thymidine uptake and immunocytochemical identification of GH-producing cells, we show that 5 nM GRF causes a 20-fold increase in the percentage of somatotrophs labeled with [3H]thymidine. The total number of somatotrophs in GRF-treated cultures was increased by 60%. Somatostatin had no measurable effect on the labeling index by itself, but it partly inhibited the GRF-induced increase in both the labeling index and the total number of cells. Forskolin caused an increase in both the percentage of somatotrophs with a [3H]thymidine-labeled nucleus and the somatotroph number similar to that caused by GRF. GH secretion as well as cellular GH content in the GRF- or forskolin-treated cells increased with culture time over the entire period, whereas secretion and content of GH gradually decreased in control or somatostatin-treated cultures during the entire culture period. These data suggest that GRF and somatostatin regulate the mitotic activity of GH-producing cells and that the effect of GRF is possibly mediated by cyclic AMP.

KW - Animals

KW - Cell Division

KW - Cells, Cultured

KW - Cyclic AMP

KW - Growth Hormone

KW - Growth Hormone-Releasing Hormone

KW - Male

KW - Pituitary Gland, Anterior

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 3018748

VL - 83

SP - 6854

EP - 6857

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 18

ER -

ID: 132900901