Fine structure of the endolymphatic duct in the rat. A scanning and transmission electron microscopy study
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Fine structure of the endolymphatic duct in the rat. A scanning and transmission electron microscopy study. / Qvortrup, K; Rostgaard, Jørgen; Bretlau, P.
In: Acta Oto-Laryngologica. Supplement, Vol. 115, No. 2, 03.1995, p. 241-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Fine structure of the endolymphatic duct in the rat. A scanning and transmission electron microscopy study
AU - Qvortrup, K
AU - Rostgaard, Jørgen
AU - Bretlau, P
PY - 1995/3
Y1 - 1995/3
N2 - To investigate the surface morphology of the endolymphatic duct epithelium, 8 rats were vascularly perfused with glutaraldehyde in a buffered and oxygenated blood substitute. Optimal preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with OsO4 and thiocarbohydrazide followed by a continuous dehydration procedure. Three types of cells were identified with the scanning electron microscope: A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, whereas in the juxta-saccular half of the duct two additional types of epithelial cells were observed. The scanning electron microscopical observations are compared and discussed with reference to transmission electron microscopical observations of the endolymphatic duct.
AB - To investigate the surface morphology of the endolymphatic duct epithelium, 8 rats were vascularly perfused with glutaraldehyde in a buffered and oxygenated blood substitute. Optimal preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with OsO4 and thiocarbohydrazide followed by a continuous dehydration procedure. Three types of cells were identified with the scanning electron microscope: A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, whereas in the juxta-saccular half of the duct two additional types of epithelial cells were observed. The scanning electron microscopical observations are compared and discussed with reference to transmission electron microscopical observations of the endolymphatic duct.
KW - Animals
KW - Ear, Inner
KW - Endolymphatic Duct
KW - Epithelial Cells
KW - Microscopy, Electron
KW - Rats
KW - Rats, Wistar
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 7610813
VL - 115
SP - 241
EP - 245
JO - Acta Oto-Laryngologica, Supplement
JF - Acta Oto-Laryngologica, Supplement
SN - 0365-5237
IS - 2
ER -
ID: 176538584