Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor
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Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor. / Raaberg, Lasse; Nexø, Ebba; Jørgensen, P E; Poulsen, Steen Seier; Jakab, M.
In: Pediatric Research, Vol. 37, No. 2, 02.1995, p. 175-181.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor
AU - Raaberg, Lasse
AU - Nexø, Ebba
AU - Jørgensen, P E
AU - Poulsen, Steen Seier
AU - Jakab, M
PY - 1995/2
Y1 - 1995/2
N2 - We have used rats with epidermal growth factor (EGF) autoantibodies to study the role of EGF deficiency during perinatal development. The study was focused on organs known to contain EGF or its receptor. Compared with controls, the offspring of autoimmune rats had a higher perinatal mortality and a lower birth weight. The weight of the lungs was particularly low in the offspring of EGF-immunized rats, and morphologically the lungs from the surviving pups seemed atelectatic and had alveolar duct dilatation, which indicates mild respiratory distress syndrome. Judged from immunohistochemical studies, the amount of surfactant protein-A was decreased, suggesting a delayed lung maturation. The offspring of EGF-immunized rats had dry and wrinkled skin. The skin was thin and the hair follicles were immature. This suggests a role for EGF in the growth and development of the skin. The liver/body weight ratio was lower in pups from EGF-immunized rats. This difference was, however, not significant (p = 0.07), but flow cytometric analyses showed a significantly lower proportion of the liver cells from newborn EGF-deficient pups to be in S-phase and indicated that these cells were larger than liver cells from controls. To study possible alterations in EGF binding, 125I-EGF was injected i.v. in newborn rats. 125I-EGF bound in all the organs investigated. The binding is listed in decreasing order: liver, gut, skin, kidney, and lungs. In the pups from EGF-immunized rats, the lungs and the skin bound a significantly higher amount than the controls.(ABSTRACT TRUNCATED AT 250 WORDS)
AB - We have used rats with epidermal growth factor (EGF) autoantibodies to study the role of EGF deficiency during perinatal development. The study was focused on organs known to contain EGF or its receptor. Compared with controls, the offspring of autoimmune rats had a higher perinatal mortality and a lower birth weight. The weight of the lungs was particularly low in the offspring of EGF-immunized rats, and morphologically the lungs from the surviving pups seemed atelectatic and had alveolar duct dilatation, which indicates mild respiratory distress syndrome. Judged from immunohistochemical studies, the amount of surfactant protein-A was decreased, suggesting a delayed lung maturation. The offspring of EGF-immunized rats had dry and wrinkled skin. The skin was thin and the hair follicles were immature. This suggests a role for EGF in the growth and development of the skin. The liver/body weight ratio was lower in pups from EGF-immunized rats. This difference was, however, not significant (p = 0.07), but flow cytometric analyses showed a significantly lower proportion of the liver cells from newborn EGF-deficient pups to be in S-phase and indicated that these cells were larger than liver cells from controls. To study possible alterations in EGF binding, 125I-EGF was injected i.v. in newborn rats. 125I-EGF bound in all the organs investigated. The binding is listed in decreasing order: liver, gut, skin, kidney, and lungs. In the pups from EGF-immunized rats, the lungs and the skin bound a significantly higher amount than the controls.(ABSTRACT TRUNCATED AT 250 WORDS)
KW - Abnormalities, Multiple
KW - Animals
KW - Autoantibodies
KW - Birth Weight
KW - Digestive System
KW - Embryonic and Fetal Development
KW - Epidermal Growth Factor
KW - Female
KW - Immunization
KW - Liver
KW - Lung
KW - Pregnancy
KW - Pregnancy Complications
KW - Pulmonary Atelectasis
KW - Rats
KW - Rats, Wistar
KW - Skin
U2 - 10.1203/00006450-199502000-00009
DO - 10.1203/00006450-199502000-00009
M3 - Journal article
C2 - 7731754
VL - 37
SP - 175
EP - 181
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 2
ER -
ID: 248364