TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans

AU - Cao, Yan Li

AU - Li, Gui Lan

AU - Wang, Kai Tuo

AU - Zhang, Hong Yin

AU - Li, Lan Fen

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Argininosuccinate lyase (ASL) is an important enzyme in arginine synthesis and the urea cycle, which are highly conserved from bacteria to eukaryotes. The gene encoding Streptococcus mutans ASL (smASL) was amplified and cloned into expression vector pET28a. The recombinant smASL protein was expressed in a soluble form in Escherichia coli strain BL21 (DE3) and purified to homogeneity by two-step column chromatography. Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5 Å. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3 Å.

AB - Argininosuccinate lyase (ASL) is an important enzyme in arginine synthesis and the urea cycle, which are highly conserved from bacteria to eukaryotes. The gene encoding Streptococcus mutans ASL (smASL) was amplified and cloned into expression vector pET28a. The recombinant smASL protein was expressed in a soluble form in Escherichia coli strain BL21 (DE3) and purified to homogeneity by two-step column chromatography. Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5 Å. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3 Å.

KW - argininosuccinate lyase

KW - Streptococcus mutans

UR - http://www.scopus.com/inward/record.url?scp=79958101763&partnerID=8YFLogxK

U2 - 10.1107/S1744309111011377

DO - 10.1107/S1744309111011377

M3 - Journal article

C2 - 21636911

AN - SCOPUS:79958101763

VL - 67

SP - 682

EP - 684

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

SN - 2053-230X

IS - 6

ER -