3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography
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3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography. / Arkill, Kp; Neal, Cr; Mantell, Jm; Michel, Cc; Qvortrup, Klaus; Rostgaard, J; Bates, DO; Knupp, C; Squire, Jm.
In: Microcirculation, Vol. 19, No. 4, 2012, p. 343-351.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - 3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography
AU - Arkill, Kp
AU - Neal, Cr
AU - Mantell, Jm
AU - Michel, Cc
AU - Qvortrup, Klaus
AU - Rostgaard, J
AU - Bates, DO
AU - Knupp, C
AU - Squire, Jm
N1 - © 2012 John Wiley & Sons Ltd.
PY - 2012
Y1 - 2012
N2 - Visualising the molecular strands making up the glycocalyx in the lumen of small blood vessels has proved to be difficult using conventional transmission electron microscopy techniques. Images obtained from tissue stained in a variety of ways have revealed a regularity in the organisation of the proteoglycan components of the glycocalyx layer (fundamental spacing about 20 nm), but require a large sample number. Attempts to visualise the glycocalyx face-on (i.e. in a direction perpendicular to the endothelial cell layer in the lumen and directly applicable for permeability modelling) has had limited success (e.g. freeze fracture). A new approach is therefore needed. Here we demonstrate the effectiveness of using the relatively novel electron microscopy technique of 3D electron tomography ontwo differently stained preparations to reveal details of the architecture of the glycocalyx just above the endothelial cell layer. One preparation uses the novel staining technique using Lanthanum Dysprosium Glycosamino Glycan adhesion (the LaDy GAGa method). © 2012 John Wiley & Sons Ltd.
AB - Visualising the molecular strands making up the glycocalyx in the lumen of small blood vessels has proved to be difficult using conventional transmission electron microscopy techniques. Images obtained from tissue stained in a variety of ways have revealed a regularity in the organisation of the proteoglycan components of the glycocalyx layer (fundamental spacing about 20 nm), but require a large sample number. Attempts to visualise the glycocalyx face-on (i.e. in a direction perpendicular to the endothelial cell layer in the lumen and directly applicable for permeability modelling) has had limited success (e.g. freeze fracture). A new approach is therefore needed. Here we demonstrate the effectiveness of using the relatively novel electron microscopy technique of 3D electron tomography ontwo differently stained preparations to reveal details of the architecture of the glycocalyx just above the endothelial cell layer. One preparation uses the novel staining technique using Lanthanum Dysprosium Glycosamino Glycan adhesion (the LaDy GAGa method). © 2012 John Wiley & Sons Ltd.
U2 - 10.1111/j.1549-8719.2012.00168.x
DO - 10.1111/j.1549-8719.2012.00168.x
M3 - Journal article
C2 - 22324320
VL - 19
SP - 343
EP - 351
JO - Microcirculation
JF - Microcirculation
SN - 1073-9688
IS - 4
ER -
ID: 37765383