GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine. / Mortensen, Kristine; Christensen, Louise Lundby; Holst, Jens Juul; Ørskov, Cathrine.

I: Regulatory Peptides, Bind 114, Nr. 2-3, 2003, s. 189-96.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mortensen, K, Christensen, LL, Holst, JJ & Ørskov, C 2003, 'GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine', Regulatory Peptides, bind 114, nr. 2-3, s. 189-96.

APA

Mortensen, K., Christensen, L. L., Holst, J. J., & Ørskov, C. (2003). GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine. Regulatory Peptides, 114(2-3), 189-96.

Vancouver

Mortensen K, Christensen LL, Holst JJ, Ørskov C. GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine. Regulatory Peptides. 2003;114(2-3):189-96.

Author

Mortensen, Kristine ; Christensen, Louise Lundby ; Holst, Jens Juul ; Ørskov, Cathrine. / GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine. I: Regulatory Peptides. 2003 ; Bind 114, Nr. 2-3. s. 189-96.

Bibtex

@article{e0f13dc074c411dbbee902004c4f4f50,
title = "GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine",
abstract = "BACKGROUND: The incretin hormones GIP and GLP-1 are thought to be produced in separate endocrine cells located in the proximal and distal ends of the mammalian small intestine, respectively. METHODS AND RESULTS: Using double immunohistochemistry and in situ hybridization, we found that GLP-1 was colocalized with either GIP or PYY in endocrine cells of the porcine, rat, and human small intestines, whereas GIP and PYY were rarely colocalized. Thus, of all the cells staining positively for either GLP-1, GIP, or both, 55-75% were GLP-1 and GIP double-stained in the mid-small intestine. Concentrations of extractable GIP and PYY were highest in the midjejunum [154 (95-167) and 141 (67-158) pmol/g, median and range, respectively], whereas GLP-1 concentrations were highest in the ileum [92 (80-207) pmol/l], but GLP-1, GIP, and PYY immunoreactive cells were found throughout the porcine small intestine. CONCLUSIONS: Our results provide a morphological basis to suggest simultaneous, rather than sequential, secretion of these hormones by postprandial luminal stimulation.",
author = "Kristine Mortensen and Christensen, {Louise Lundby} and Holst, {Jens Juul} and Cathrine {\O}rskov",
note = "Keywords: Animals; Chromatography, Gel; Endocrine Glands; Female; Gastric Inhibitory Polypeptide; Glucagon; Glucagon-Like Peptide 1; In Situ Hybridization; Intestine, Small; Peptide Fragments; Protein Precursors; Swine",
year = "2003",
language = "English",
volume = "114",
pages = "189--96",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "2-3",

}

RIS

TY - JOUR

T1 - GLP-1 and GIP are colocalized in a subset of endocrine cells in the small intestine

AU - Mortensen, Kristine

AU - Christensen, Louise Lundby

AU - Holst, Jens Juul

AU - Ørskov, Cathrine

N1 - Keywords: Animals; Chromatography, Gel; Endocrine Glands; Female; Gastric Inhibitory Polypeptide; Glucagon; Glucagon-Like Peptide 1; In Situ Hybridization; Intestine, Small; Peptide Fragments; Protein Precursors; Swine

PY - 2003

Y1 - 2003

N2 - BACKGROUND: The incretin hormones GIP and GLP-1 are thought to be produced in separate endocrine cells located in the proximal and distal ends of the mammalian small intestine, respectively. METHODS AND RESULTS: Using double immunohistochemistry and in situ hybridization, we found that GLP-1 was colocalized with either GIP or PYY in endocrine cells of the porcine, rat, and human small intestines, whereas GIP and PYY were rarely colocalized. Thus, of all the cells staining positively for either GLP-1, GIP, or both, 55-75% were GLP-1 and GIP double-stained in the mid-small intestine. Concentrations of extractable GIP and PYY were highest in the midjejunum [154 (95-167) and 141 (67-158) pmol/g, median and range, respectively], whereas GLP-1 concentrations were highest in the ileum [92 (80-207) pmol/l], but GLP-1, GIP, and PYY immunoreactive cells were found throughout the porcine small intestine. CONCLUSIONS: Our results provide a morphological basis to suggest simultaneous, rather than sequential, secretion of these hormones by postprandial luminal stimulation.

AB - BACKGROUND: The incretin hormones GIP and GLP-1 are thought to be produced in separate endocrine cells located in the proximal and distal ends of the mammalian small intestine, respectively. METHODS AND RESULTS: Using double immunohistochemistry and in situ hybridization, we found that GLP-1 was colocalized with either GIP or PYY in endocrine cells of the porcine, rat, and human small intestines, whereas GIP and PYY were rarely colocalized. Thus, of all the cells staining positively for either GLP-1, GIP, or both, 55-75% were GLP-1 and GIP double-stained in the mid-small intestine. Concentrations of extractable GIP and PYY were highest in the midjejunum [154 (95-167) and 141 (67-158) pmol/g, median and range, respectively], whereas GLP-1 concentrations were highest in the ileum [92 (80-207) pmol/l], but GLP-1, GIP, and PYY immunoreactive cells were found throughout the porcine small intestine. CONCLUSIONS: Our results provide a morphological basis to suggest simultaneous, rather than sequential, secretion of these hormones by postprandial luminal stimulation.

M3 - Journal article

C2 - 12832109

VL - 114

SP - 189

EP - 196

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 2-3

ER -

ID: 121344