Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique

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Standard

Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique. / Kulahin, Nikolaj; Grunnet, Lars Groth; Lundh, Morten; Christensen, Dan Ploug; Jorgensen, Rasmus; Heding, Anders; Billestrup, Nils; Berezin, Vladimir; Bock, Elisabeth; Mandrup-Poulsen, Thomas.

I: FEBS Letters, Bind 585, Nr. 1, 03.01.2011, s. 58-64.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kulahin, N, Grunnet, LG, Lundh, M, Christensen, DP, Jorgensen, R, Heding, A, Billestrup, N, Berezin, V, Bock, E & Mandrup-Poulsen, T 2011, 'Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique', FEBS Letters, bind 585, nr. 1, s. 58-64. https://doi.org/10.1016/j.febslet.2010.11.043

APA

Kulahin, N., Grunnet, L. G., Lundh, M., Christensen, D. P., Jorgensen, R., Heding, A., Billestrup, N., Berezin, V., Bock, E., & Mandrup-Poulsen, T. (2011). Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique. FEBS Letters, 585(1), 58-64. https://doi.org/10.1016/j.febslet.2010.11.043

Vancouver

Kulahin N, Grunnet LG, Lundh M, Christensen DP, Jorgensen R, Heding A o.a. Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique. FEBS Letters. 2011 jan. 3;585(1):58-64. https://doi.org/10.1016/j.febslet.2010.11.043

Author

Kulahin, Nikolaj ; Grunnet, Lars Groth ; Lundh, Morten ; Christensen, Dan Ploug ; Jorgensen, Rasmus ; Heding, Anders ; Billestrup, Nils ; Berezin, Vladimir ; Bock, Elisabeth ; Mandrup-Poulsen, Thomas. / Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique. I: FEBS Letters. 2011 ; Bind 585, Nr. 1. s. 58-64.

Bibtex

@article{e75f28495e1e456c969470a0d7ec718c,
title = "Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique",
abstract = "Biological activity of the neural cell adhesion molecule (NCAM) depends on both adhesion and activation of intra-cellular signaling. Based on in vitro experiments with truncated extra-cellular domains, several models describing homophilic NCAM trans- and cis-interactions have been proposed. However, cis-dimerization in living cells has not been shown directly and the role of the cytoplasmic part in NCAM dimerization is poorly understood. Here, we used the bioluminescence resonance energy transfer (BRET(2)) technique to directly demonstrate that full-length NCAM cis-homodimerizes in living cells. Based on BRET(2)50 values we suggest that the intra-cellular part of NCAM inhibits cis-dimerization, an effect mainly dependent on the palmitoylation sites.",
keywords = "Animals, COS Cells, Cercopithecus aethiops, Chemiluminescent Measurements, Energy Transfer, Green Fluorescent Proteins, Humans, Lipoylation, Neural Cell Adhesion Molecules, Protein Multimerization, Recombinant Fusion Proteins, Transfection",
author = "Nikolaj Kulahin and Grunnet, {Lars Groth} and Morten Lundh and Christensen, {Dan Ploug} and Rasmus Jorgensen and Anders Heding and Nils Billestrup and Vladimir Berezin and Elisabeth Bock and Thomas Mandrup-Poulsen",
note = "Copyright {\textcopyright} 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",
year = "2011",
month = jan,
day = "3",
doi = "10.1016/j.febslet.2010.11.043",
language = "English",
volume = "585",
pages = "58--64",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Direct demonstration of NCAM cis-dimerization and inhibitory effect of palmitoylation using the BRET2 technique

AU - Kulahin, Nikolaj

AU - Grunnet, Lars Groth

AU - Lundh, Morten

AU - Christensen, Dan Ploug

AU - Jorgensen, Rasmus

AU - Heding, Anders

AU - Billestrup, Nils

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Mandrup-Poulsen, Thomas

N1 - Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

PY - 2011/1/3

Y1 - 2011/1/3

N2 - Biological activity of the neural cell adhesion molecule (NCAM) depends on both adhesion and activation of intra-cellular signaling. Based on in vitro experiments with truncated extra-cellular domains, several models describing homophilic NCAM trans- and cis-interactions have been proposed. However, cis-dimerization in living cells has not been shown directly and the role of the cytoplasmic part in NCAM dimerization is poorly understood. Here, we used the bioluminescence resonance energy transfer (BRET(2)) technique to directly demonstrate that full-length NCAM cis-homodimerizes in living cells. Based on BRET(2)50 values we suggest that the intra-cellular part of NCAM inhibits cis-dimerization, an effect mainly dependent on the palmitoylation sites.

AB - Biological activity of the neural cell adhesion molecule (NCAM) depends on both adhesion and activation of intra-cellular signaling. Based on in vitro experiments with truncated extra-cellular domains, several models describing homophilic NCAM trans- and cis-interactions have been proposed. However, cis-dimerization in living cells has not been shown directly and the role of the cytoplasmic part in NCAM dimerization is poorly understood. Here, we used the bioluminescence resonance energy transfer (BRET(2)) technique to directly demonstrate that full-length NCAM cis-homodimerizes in living cells. Based on BRET(2)50 values we suggest that the intra-cellular part of NCAM inhibits cis-dimerization, an effect mainly dependent on the palmitoylation sites.

KW - Animals

KW - COS Cells

KW - Cercopithecus aethiops

KW - Chemiluminescent Measurements

KW - Energy Transfer

KW - Green Fluorescent Proteins

KW - Humans

KW - Lipoylation

KW - Neural Cell Adhesion Molecules

KW - Protein Multimerization

KW - Recombinant Fusion Proteins

KW - Transfection

U2 - 10.1016/j.febslet.2010.11.043

DO - 10.1016/j.febslet.2010.11.043

M3 - Journal article

C2 - 21115007

VL - 585

SP - 58

EP - 64

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1

ER -

ID: 33903163