Ultrastructure of the epithelial cells of the endolymphatic duct in the rat
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Ultrastructure of the epithelial cells of the endolymphatic duct in the rat. / Qvortrup, K; Rostgaard, J.
In: Acta Oto-Laryngologica. Supplement, Vol. 114, No. 1, 01.1994, p. 30-9.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Ultrastructure of the epithelial cells of the endolymphatic duct in the rat
AU - Qvortrup, K
AU - Rostgaard, J
PY - 1994/1
Y1 - 1994/1
N2 - The ultrastructure of the epithelial cells of the endolymphatic duct in the rat is described, following vascular perfusion-fixation of live, anaesthetised and artificially respirated animals. The animals were fixed by means of a pressure feed-back controlled peristaltic pump and an isotonic perfusate-fixative containing glutaraldehyde and Dextran. The endolymphatic duct was isolated by microdissection after the perfusion-fixation, to omit the step of a demineralization procedure. The proximal, intermediate and juxta-saccular parts of the endolymphatic duct were embedded, sectioned and studied separately in the electron microscope. Postfixation in a solution containing OsO4 and potassium ferricyanide revealed a well-developed tubulo-cisternal endoplasmic reticulum (TER), not previously described. Serial sectioning and computerized three-dimensional reconstruction demonstrated a continuity of the TER through the cell from subsurface cisterns abutting on the apical cell membrane to subsurface cisterns abutting on the basolateral cell membrane. The TER resembles that found in solute transporting epithelia, e.g., renal proximal tubule, gall bladder, small intestine and choroid plexus. A fluid resorptive capacity of the epithelial cells of the endolymphatic duct is compatible with the fine structure revealed in the present study. Epithelial cells in the juxta-saccular part of the duct display morphological indications of a secretory activity; furthermore, multivesicular bodies were observed in the epithelial cells throughout the endolymphatic duct.
AB - The ultrastructure of the epithelial cells of the endolymphatic duct in the rat is described, following vascular perfusion-fixation of live, anaesthetised and artificially respirated animals. The animals were fixed by means of a pressure feed-back controlled peristaltic pump and an isotonic perfusate-fixative containing glutaraldehyde and Dextran. The endolymphatic duct was isolated by microdissection after the perfusion-fixation, to omit the step of a demineralization procedure. The proximal, intermediate and juxta-saccular parts of the endolymphatic duct were embedded, sectioned and studied separately in the electron microscope. Postfixation in a solution containing OsO4 and potassium ferricyanide revealed a well-developed tubulo-cisternal endoplasmic reticulum (TER), not previously described. Serial sectioning and computerized three-dimensional reconstruction demonstrated a continuity of the TER through the cell from subsurface cisterns abutting on the apical cell membrane to subsurface cisterns abutting on the basolateral cell membrane. The TER resembles that found in solute transporting epithelia, e.g., renal proximal tubule, gall bladder, small intestine and choroid plexus. A fluid resorptive capacity of the epithelial cells of the endolymphatic duct is compatible with the fine structure revealed in the present study. Epithelial cells in the juxta-saccular part of the duct display morphological indications of a secretory activity; furthermore, multivesicular bodies were observed in the epithelial cells throughout the endolymphatic duct.
KW - Animals
KW - Basement Membrane
KW - Cell Membrane
KW - Cilia
KW - Cytoplasm
KW - Desmosomes
KW - Endolymphatic Duct
KW - Endolymphatic Sac
KW - Endoplasmic Reticulum
KW - Epithelium
KW - Glycogen
KW - Golgi Apparatus
KW - Intercellular Junctions
KW - Microscopy, Electron
KW - Microtubules
KW - Microvilli
KW - Rats
KW - Rats, Wistar
KW - Saccule and Utricle
KW - Vacuoles
KW - Vestibular Aqueduct
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 8128850
VL - 114
SP - 30
EP - 39
JO - Acta Oto-Laryngologica, Supplement
JF - Acta Oto-Laryngologica, Supplement
SN - 0365-5237
IS - 1
ER -
ID: 176771347