The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
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The polycomb group protein Suz12 is required for embryonic stem cell differentiation. / Pasini, Diego; Bracken, Adrian P; Hansen, Jacob Bo Højberg; Capillo, Manuela; Helin, Kristian.
In: Molecular and Cellular Biology, Vol. 27, No. 10, 2007, p. 3769-79.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
AU - Pasini, Diego
AU - Bracken, Adrian P
AU - Hansen, Jacob Bo Højberg
AU - Capillo, Manuela
AU - Helin, Kristian
N1 - Keywords: Animals; Cell Differentiation; Cells, Cultured; Chromatin Immunoprecipitation; DNA-Binding Proteins; Embryonic Stem Cells; Gene Expression Regulation, Developmental; Histones; Homeodomain Proteins; Lysine; Methylation; Mice; Mice, Knockout; Multiprotein Complexes; Neurons; Octamer Transcription Factor-3; Promoter Regions (Genetics); Repressor Proteins; Transcription, Genetic
PY - 2007
Y1 - 2007
N2 - Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity and its inactivation results in early lethality of mouse embryos. Here, we demonstrate that Suz12(-/-) mouse embryonic stem (ES) cells can be established and expanded in tissue culture. The Suz12(-/-) ES cells are characterized by global loss of H3K27 trimethylation (H3K27me3) and higher expression levels of differentiation-specific genes. Moreover, Suz12(-/-) ES cells are impaired in proper differentiation, resulting in a lack of repression of ES cell markers as well as activation of differentiation-specific genes. Finally, we demonstrate that the PcGs are actively recruited to several genes during ES cell differentiation, which despite an increase in H3K27me3 levels is not always sufficient to prevent transcriptional activation. In summary, we demonstrate that Suz12 is required for the establishment of specific expression programs required for ES cell differentiation. Furthermore, we provide evidence that PcGs have different mechanisms to regulate transcription during cellular differentiation.
AB - Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity and its inactivation results in early lethality of mouse embryos. Here, we demonstrate that Suz12(-/-) mouse embryonic stem (ES) cells can be established and expanded in tissue culture. The Suz12(-/-) ES cells are characterized by global loss of H3K27 trimethylation (H3K27me3) and higher expression levels of differentiation-specific genes. Moreover, Suz12(-/-) ES cells are impaired in proper differentiation, resulting in a lack of repression of ES cell markers as well as activation of differentiation-specific genes. Finally, we demonstrate that the PcGs are actively recruited to several genes during ES cell differentiation, which despite an increase in H3K27me3 levels is not always sufficient to prevent transcriptional activation. In summary, we demonstrate that Suz12 is required for the establishment of specific expression programs required for ES cell differentiation. Furthermore, we provide evidence that PcGs have different mechanisms to regulate transcription during cellular differentiation.
U2 - 10.1128/MCB.01432-06
DO - 10.1128/MCB.01432-06
M3 - Journal article
C2 - 17339329
VL - 27
SP - 3769
EP - 3779
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 10
ER -
ID: 5035264