The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing
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The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing. / Redstone, Heather A; Buie, William D; Hart, David A; Wallace, Laurie; Hornby, Pamela J; Sague, Sarah; Holst, Jens Juul; Sigalet, David L.
In: Gastroenterology Research and Practice, Vol. 2010, 01.01.2010, p. Article ID 672453.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing
AU - Redstone, Heather A
AU - Buie, William D
AU - Hart, David A
AU - Wallace, Laurie
AU - Hornby, Pamela J
AU - Sague, Sarah
AU - Holst, Jens Juul
AU - Sigalet, David L
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Background. Glucagon-like peptide 2 (GLP-2) is an intestinal specific trophic hormone, with therapeutic potential; the effects on intestinal healing are unknown. We used a rat model of colonic healing, under normoxic, and stress (hypoxic) conditions to examine the effect of GLP-2 on intestinal healing. Methods. Following colonic transection and reanastomosis, animals were randomized to one of six groups (n = 8/group): controls, native GLP-2, long-acting GLP-2 (GLP-2- MIMETIBODY, GLP-2-MMB), animals were housed under normoxic or hypoxic (11%¿¿O(2)) conditions. Animals were studied five days post-operation for anastomotic strength and wound characteristics. Results. Anastomotic bursting pressure was unchanged by GLP-2 or GLP-2-MMB in normoxic or hypoxic animals; both treatments increased crypt cell proliferation. Wound IL-1ß increased with GLP-2; IFN¿ with GLP-2 and GLP-2-MMB. IL-10 and TGF-ß were decreased; Type I collagen mRNA expression increased in hypoxic animals while Type III collagen was reduced with both GLP-2 agonists. GLP-2 MMB, but not native GLP-2 increased TIMP 1-3 mRNA levels in hypoxia. Conclusions. The effects on CCP, cytokines and wound healing were similar for both GLP-2 agonists under normoxic and hypoxic conditions; anastomotic strength was not affected. This suggests that GLP-2 (or agonists) could be safely used peri-operatively; direct studies will be required.
AB - Background. Glucagon-like peptide 2 (GLP-2) is an intestinal specific trophic hormone, with therapeutic potential; the effects on intestinal healing are unknown. We used a rat model of colonic healing, under normoxic, and stress (hypoxic) conditions to examine the effect of GLP-2 on intestinal healing. Methods. Following colonic transection and reanastomosis, animals were randomized to one of six groups (n = 8/group): controls, native GLP-2, long-acting GLP-2 (GLP-2- MIMETIBODY, GLP-2-MMB), animals were housed under normoxic or hypoxic (11%¿¿O(2)) conditions. Animals were studied five days post-operation for anastomotic strength and wound characteristics. Results. Anastomotic bursting pressure was unchanged by GLP-2 or GLP-2-MMB in normoxic or hypoxic animals; both treatments increased crypt cell proliferation. Wound IL-1ß increased with GLP-2; IFN¿ with GLP-2 and GLP-2-MMB. IL-10 and TGF-ß were decreased; Type I collagen mRNA expression increased in hypoxic animals while Type III collagen was reduced with both GLP-2 agonists. GLP-2 MMB, but not native GLP-2 increased TIMP 1-3 mRNA levels in hypoxia. Conclusions. The effects on CCP, cytokines and wound healing were similar for both GLP-2 agonists under normoxic and hypoxic conditions; anastomotic strength was not affected. This suggests that GLP-2 (or agonists) could be safely used peri-operatively; direct studies will be required.
U2 - 10.1155/2010/672453
DO - 10.1155/2010/672453
M3 - Journal article
C2 - 20953406
VL - 2010
SP - Article ID 672453
JO - Gastroenterology Research and Practice
JF - Gastroenterology Research and Practice
SN - 1687-6121
ER -
ID: 33938905