Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin
Research output: Contribution to journal › Journal article › Research › peer-review
Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.
Original language | English |
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Journal | International Journal of Peptide and Protein Research |
Volume | 37 |
Issue number | 3 |
Pages (from-to) | 185-90 |
Number of pages | 6 |
Publication status | Published - Mar 1991 |
- Amino Acid Sequence, Animals, Chromatography, High Pressure Liquid, Cyclic AMP, Insulin, Mice, Molecular Sequence Data, Radioimmunoassay, Secretin, Swine
Research areas
ID: 45574735