Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin. / Kofod, Hans.

In: International Journal of Peptide and Protein Research, Vol. 37, No. 3, 03.1991, p. 185-90.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kofod, H 1991, 'Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin', International Journal of Peptide and Protein Research, vol. 37, no. 3, pp. 185-90.

APA

Kofod, H. (1991). Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin. International Journal of Peptide and Protein Research, 37(3), 185-90.

Vancouver

Kofod H. Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin. International Journal of Peptide and Protein Research. 1991 Mar;37(3):185-90.

Author

Kofod, Hans. / Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin. In: International Journal of Peptide and Protein Research. 1991 ; Vol. 37, No. 3. pp. 185-90.

Bibtex

@article{33cf963997fc46ea8fdb8f08a424ba13,
title = "Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin",
abstract = "Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.",
keywords = "Amino Acid Sequence, Animals, Chromatography, High Pressure Liquid, Cyclic AMP, Insulin, Mice, Molecular Sequence Data, Radioimmunoassay, Secretin, Swine",
author = "Hans Kofod",
year = "1991",
month = mar,
language = "English",
volume = "37",
pages = "185--90",
journal = "International Journal of Peptide and Protein Research",
number = "3",

}

RIS

TY - JOUR

T1 - Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin

AU - Kofod, Hans

PY - 1991/3

Y1 - 1991/3

N2 - Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.

AB - Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.

KW - Amino Acid Sequence

KW - Animals

KW - Chromatography, High Pressure Liquid

KW - Cyclic AMP

KW - Insulin

KW - Mice

KW - Molecular Sequence Data

KW - Radioimmunoassay

KW - Secretin

KW - Swine

M3 - Journal article

C2 - 1651289

VL - 37

SP - 185

EP - 190

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

IS - 3

ER -

ID: 45574735