Strength training increases the size of the satellite cell pool in type I and II fibres of chronically painful trapezius muscle in females
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Strength training increases the size of the satellite cell pool in type I and II fibres of chronically painful trapezius muscle in females. / Mackey, Abigail; Andersen, Lars L; Frandsen, Ulrik; Sjøgaard, Gisela.
In: Journal of Physiology, Vol. 589, No. Pt 22, 15.11.2011, p. 5503-15.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Strength training increases the size of the satellite cell pool in type I and II fibres of chronically painful trapezius muscle in females
AU - Mackey, Abigail
AU - Andersen, Lars L
AU - Frandsen, Ulrik
AU - Sjøgaard, Gisela
PY - 2011/11/15
Y1 - 2011/11/15
N2 - While strength training has been shown to be effective in mediating hypertrophy and reducing pain in trapezius myalgia, responses at the cellular level have not previously been studied. This study investigated the potential of strength training targeting the affected muscles (SST, n = 18) and general fitness training (GFT, n = 16) to augment the satellite cell (SC) and macrophage pools in the trapezius muscles of women diagnosed with trapezius myalgia. A group receiving general health information (REF, n = 8) served as a control. Muscle biopsies were collected from the trapezius muscles of the 42 women (age 44 ± 8 years; mean ± SD) before and after the 10 week intervention period and were analysed by immunohistochemistry for SCs, macrophages and myonuclei. The SC content of type I and II fibres was observed to increase significantly from baseline by 65% and 164%, respectively, with SST (P <0.0001), together with a significant correlation between the baseline number of SCs and the extent of hypertrophy (r = -0.669, P = 0.005). SST also resulted in a 74% enhancement of the trapezius macrophage content (P <0.01), accompanied by evidence for the presence of an increased number of actively dividing cells (Ki67(+)) post-SST (P <0.001). GFT resulted in a significant 23% increase in the SC content of type II fibres, when expressed relative to myonuclear number only (P <0.05). No changes in the number of myonuclei per fibre or myonuclear domain were detected in any group. These findings provide strong support at the cellular level for the potential of SST to induce a strong myogenic response in this population.
AB - While strength training has been shown to be effective in mediating hypertrophy and reducing pain in trapezius myalgia, responses at the cellular level have not previously been studied. This study investigated the potential of strength training targeting the affected muscles (SST, n = 18) and general fitness training (GFT, n = 16) to augment the satellite cell (SC) and macrophage pools in the trapezius muscles of women diagnosed with trapezius myalgia. A group receiving general health information (REF, n = 8) served as a control. Muscle biopsies were collected from the trapezius muscles of the 42 women (age 44 ± 8 years; mean ± SD) before and after the 10 week intervention period and were analysed by immunohistochemistry for SCs, macrophages and myonuclei. The SC content of type I and II fibres was observed to increase significantly from baseline by 65% and 164%, respectively, with SST (P <0.0001), together with a significant correlation between the baseline number of SCs and the extent of hypertrophy (r = -0.669, P = 0.005). SST also resulted in a 74% enhancement of the trapezius macrophage content (P <0.01), accompanied by evidence for the presence of an increased number of actively dividing cells (Ki67(+)) post-SST (P <0.001). GFT resulted in a significant 23% increase in the SC content of type II fibres, when expressed relative to myonuclear number only (P <0.05). No changes in the number of myonuclei per fibre or myonuclear domain were detected in any group. These findings provide strong support at the cellular level for the potential of SST to induce a strong myogenic response in this population.
KW - Adult
KW - Chronic Pain
KW - Female
KW - Humans
KW - Hypertrophy
KW - Ki-67 Antigen
KW - Laminin
KW - Macrophages
KW - Middle Aged
KW - Muscle Fibers, Fast-Twitch
KW - Muscle Fibers, Slow-Twitch
KW - Muscular Diseases
KW - Musculoskeletal Pain
KW - Myosin Type I
KW - Neck Muscles
KW - PAX7 Transcription Factor
KW - Resistance Training
KW - Satellite Cells, Skeletal Muscle
U2 - 10.1113/jphysiol.2011.217885
DO - 10.1113/jphysiol.2011.217885
M3 - Journal article
C2 - 21946848
VL - 589
SP - 5503
EP - 5515
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - Pt 22
ER -
ID: 47292603