Stage-specific control of neural crest stem cell proliferation by the small rho GTPases Cdc42 and Rac1
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Stage-specific control of neural crest stem cell proliferation by the small rho GTPases Cdc42 and Rac1. / Fuchs, Sebastian; Herzog, Dominik; Sumara, Grzegorz; Büchmann-Møller, Stine; Civenni, Gianluca; Wu, Xunwei; Chrostek-Grashoff, Anna; Suter, Ueli; Ricci, Romeo; Relvas, João B; Brakebusch, Cord; Sommer, Lukas.
In: Cell Stem Cell, Vol. 4, No. 3, 2009, p. 236-47.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stage-specific control of neural crest stem cell proliferation by the small rho GTPases Cdc42 and Rac1
AU - Fuchs, Sebastian
AU - Herzog, Dominik
AU - Sumara, Grzegorz
AU - Büchmann-Møller, Stine
AU - Civenni, Gianluca
AU - Wu, Xunwei
AU - Chrostek-Grashoff, Anna
AU - Suter, Ueli
AU - Ricci, Romeo
AU - Relvas, João B
AU - Brakebusch, Cord
AU - Sommer, Lukas
N1 - Keywords: Alleles; Animals; Cell Cycle; Cell Differentiation; Cell Movement; Cell Proliferation; Epidermal Growth Factor; Gene Deletion; Mice; Mice, Knockout; Neural Crest; Recombination, Genetic; Stem Cells; cdc42 GTP-Binding Protein; rac1 GTP-Binding Protein
PY - 2009
Y1 - 2009
N2 - The neural crest (NC) generates a variety of neural and non-neural tissues during vertebrate development. Both migratory NC cells and their target structures contain cells with stem cell features. Here we show that these populations of neural crest-derived stem cells (NCSCs) are differentially regulated by small Rho GTPases. Deletion of either Cdc42 or Rac1 in the NC results in size reduction of multiple NC target structures because of increased cell-cycle exit, while NC cells emigrating from the neural tube are not affected. Consistently, Cdc42 or Rac1 inactivation reduces self-renewal and proliferation of later stage, but not early migratory NCSCs. This stage-specific requirement for small Rho GTPases is due to changes in NCSCs that, during development, acquire responsiveness to mitogenic EGF acting upstream of both Cdc42 and Rac1. Thus, our data reveal distinct mechanisms for growth control of NCSCs from different developmental stages.
AB - The neural crest (NC) generates a variety of neural and non-neural tissues during vertebrate development. Both migratory NC cells and their target structures contain cells with stem cell features. Here we show that these populations of neural crest-derived stem cells (NCSCs) are differentially regulated by small Rho GTPases. Deletion of either Cdc42 or Rac1 in the NC results in size reduction of multiple NC target structures because of increased cell-cycle exit, while NC cells emigrating from the neural tube are not affected. Consistently, Cdc42 or Rac1 inactivation reduces self-renewal and proliferation of later stage, but not early migratory NCSCs. This stage-specific requirement for small Rho GTPases is due to changes in NCSCs that, during development, acquire responsiveness to mitogenic EGF acting upstream of both Cdc42 and Rac1. Thus, our data reveal distinct mechanisms for growth control of NCSCs from different developmental stages.
U2 - 10.1016/j.stem.2009.01.017
DO - 10.1016/j.stem.2009.01.017
M3 - Journal article
C2 - 19265663
VL - 4
SP - 236
EP - 247
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 3
ER -
ID: 12866310