Small molecule screening identifies targetable zebrafish pigmentation pathways
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Small molecule screening identifies targetable zebrafish pigmentation pathways. / Colanesi, Sarah; Taylor, Kerrie L; Temperley, Nicholas D; Lundegaard, Pia R; Liu, Dong; North, Trista E; Ishizaki, Hironori; Kelsh, Robert N; Patton, E Elizabeth.
In: Pigment Cell & Melanoma Research, Vol. 25, No. 2, 03.2012, p. 131-43.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Small molecule screening identifies targetable zebrafish pigmentation pathways
AU - Colanesi, Sarah
AU - Taylor, Kerrie L
AU - Temperley, Nicholas D
AU - Lundegaard, Pia R
AU - Liu, Dong
AU - North, Trista E
AU - Ishizaki, Hironori
AU - Kelsh, Robert N
AU - Patton, E Elizabeth
N1 - © 2012 John Wiley & Sons A/S.
PY - 2012/3
Y1 - 2012/3
N2 - Small molecules complement genetic mutants and can be used to probe pigment cell biology by inhibiting specific proteins or pathways. Here, we present the results of a screen of active compounds for those that affect the processes of melanocyte and iridophore development in zebrafish and investigate the effects of a few of these compounds in further detail. We identified and confirmed 57 compounds that altered pigment cell patterning, number, survival, or differentiation. Additional tissue targets and toxicity of small molecules are also discussed. Given that the majority of cell types, including pigment cells, are conserved between zebrafish and other vertebrates, we present these chemicals as molecular tools to study developmental processes of pigment cells in living animals and emphasize the value of zebrafish as an in vivo system for testing the on- and off-target activities of clinically active drugs.
AB - Small molecules complement genetic mutants and can be used to probe pigment cell biology by inhibiting specific proteins or pathways. Here, we present the results of a screen of active compounds for those that affect the processes of melanocyte and iridophore development in zebrafish and investigate the effects of a few of these compounds in further detail. We identified and confirmed 57 compounds that altered pigment cell patterning, number, survival, or differentiation. Additional tissue targets and toxicity of small molecules are also discussed. Given that the majority of cell types, including pigment cells, are conserved between zebrafish and other vertebrates, we present these chemicals as molecular tools to study developmental processes of pigment cells in living animals and emphasize the value of zebrafish as an in vivo system for testing the on- and off-target activities of clinically active drugs.
KW - Animals
KW - Cell Count
KW - Chromatophores
KW - Cyclooxygenase Inhibitors
KW - Drug Evaluation, Preclinical
KW - Embryo, Nonmammalian
KW - Heterocyclic Compounds, 3-Ring
KW - Melanocytes
KW - Metabolic Networks and Pathways
KW - Phenotype
KW - Pigmentation
KW - Purines
KW - Pyrimidinones
KW - Small Molecule Libraries
KW - Tyrphostins
KW - Zebrafish
U2 - 10.1111/j.1755-148X.2012.00977.x
DO - 10.1111/j.1755-148X.2012.00977.x
M3 - Journal article
C2 - 22252091
VL - 25
SP - 131
EP - 143
JO - Pigment Cell & Melanoma Research
JF - Pigment Cell & Melanoma Research
SN - 1755-1471
IS - 2
ER -
ID: 154564661