Role of laminin receptor in tumor cell migration.
Research output: Contribution to journal › Journal article › Research › peer-review
Polyclonal antisera were made against biochemically purified laminin receptor protein as well as against synthetic peptides deduced from a complementary DNA clone corresponding to the COOH-terminal end of the laminin receptor (U.M. Wewer et al., Proc. Natl. Acad. Sci. USA, 83: 7137-7141, 1986). These antisera were used to study the potential role of laminin receptor in laminin-mediated attachment and haptotactic migration of human A2058 melanoma cells. The anti-laminin receptor antisera reacted with the surface of suspended, nonpermeabilized melanoma and carcinoma cells. The anti-laminin receptor antisera blocked the surface interaction of A2058 cells with endogenous laminin, resulting in the inhibition of laminin-mediated cell attachment. The A2058 melanoma cells migrated toward a gradient of solid phase laminin or fibronectin (haptotaxis). Anti-laminin antiserum abolished haptotaxis on laminin but not on fibronectin. Synthetic peptide GRGDS corresponding to the fibronectin cell-binding domain inhibited haptotaxis on fibronectin but not on laminin. Both types of anti-laminin receptor antisera inhibited haptotaxis on laminin but not on fibronectin. Using immunohistochemistry, invading human carcinoma cells in vivo exhibited a marked cytoplasmic immunoreactivity for the receptor antigen. Together these findings indicate a specific role for the laminin receptor in laminin-mediated migration and that the ligand binding of the laminin receptor is encompassed in the COOH-terminal end of the protein.
Original language | English |
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Journal | Cancer Research |
Volume | 47 |
Issue number | 21 |
Pages (from-to) | 5691-8 |
Number of pages | 7 |
ISSN | 0008-5472 |
Publication status | Published - 1987 |
Bibliographical note
Keywords: Cell Adhesion; Cell Movement; Enzyme-Linked Immunosorbent Assay; Fibronectins; Humans; Immune Sera; Laminin; Melanoma; Neoplasm Invasiveness; Neoplasms; Receptors, Immunologic; Receptors, Laminin
ID: 5236924