RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism. / García-Mariscal, Alberto; Peyrollier, Karine; Basse, Astrid; Pedersen, Esben Ditlev Kølle; Rühl, Ralph; van Hengel, Jolanda; Brakebusch, Cord.

In: Small GTPases, Vol. 9, No. 5, 2018, p. 433–444.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

García-Mariscal, A, Peyrollier, K, Basse, A, Pedersen, EDK, Rühl, R, van Hengel, J & Brakebusch, C 2018, 'RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism', Small GTPases, vol. 9, no. 5, pp. 433–444. https://doi.org/10.1080/21541248.2016.1248272

APA

García-Mariscal, A., Peyrollier, K., Basse, A., Pedersen, E. D. K., Rühl, R., van Hengel, J., & Brakebusch, C. (2018). RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism. Small GTPases, 9(5), 433–444. https://doi.org/10.1080/21541248.2016.1248272

Vancouver

García-Mariscal A, Peyrollier K, Basse A, Pedersen EDK, Rühl R, van Hengel J et al. RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism. Small GTPases. 2018;9(5):433–444. https://doi.org/10.1080/21541248.2016.1248272

Author

García-Mariscal, Alberto ; Peyrollier, Karine ; Basse, Astrid ; Pedersen, Esben Ditlev Kølle ; Rühl, Ralph ; van Hengel, Jolanda ; Brakebusch, Cord. / RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism. In: Small GTPases. 2018 ; Vol. 9, No. 5. pp. 433–444.

Bibtex

@article{2f3ab5d3d123469fb63583b55b63749a,
title = "RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism",
abstract = "The ubiquitously expressed small GTPase RhoA is essential for embryonic development and mutated in different cancers. Functionally, it is well described as a regulator of the actin cytoskeleton, but its role in gene regulation is less understood. Using primary mouse keratinocytes with a deletion of the RhoA gene, we have now been exploring how the loss of RhoA affects gene expression. Performing transcription factor reporter assays, we found a significantly decreased activity of a RAR luciferase reporter in RhoA-null keratinocytes. Inhibition of the RhoA effector ROCK in control cells reproduced this phenotype. ATRA and retinal, but not retinol increased RAR reporter activity of keratinocytes with impaired RhoA/ROCK signalling, suggesting that retinol metabolism is regulated by RhoA/ROCK signalling. Furthermore a significant percentage of known ATRA target genes displayed altered expression in RhoA-null keratinocytes. These data reveal an unexpected link between the cytoskeletal regulator RhoA and retinoid signalling and uncover a novel pathway by which RhoA regulates gene expression.",
author = "Alberto Garc{\'i}a-Mariscal and Karine Peyrollier and Astrid Basse and Pedersen, {Esben Ditlev K{\o}lle} and Ralph R{\"u}hl and {van Hengel}, Jolanda and Cord Brakebusch",
year = "2018",
doi = "10.1080/21541248.2016.1248272",
language = "English",
volume = "9",
pages = "433–444",
journal = "Small GTPases",
issn = "2154-1248",
publisher = "Taylor & Francis",
number = "5",

}

RIS

TY - JOUR

T1 - RhoA Controls Retinoid Signaling by ROCK Dependent Regulation of Retinol Metabolism

AU - García-Mariscal, Alberto

AU - Peyrollier, Karine

AU - Basse, Astrid

AU - Pedersen, Esben Ditlev Kølle

AU - Rühl, Ralph

AU - van Hengel, Jolanda

AU - Brakebusch, Cord

PY - 2018

Y1 - 2018

N2 - The ubiquitously expressed small GTPase RhoA is essential for embryonic development and mutated in different cancers. Functionally, it is well described as a regulator of the actin cytoskeleton, but its role in gene regulation is less understood. Using primary mouse keratinocytes with a deletion of the RhoA gene, we have now been exploring how the loss of RhoA affects gene expression. Performing transcription factor reporter assays, we found a significantly decreased activity of a RAR luciferase reporter in RhoA-null keratinocytes. Inhibition of the RhoA effector ROCK in control cells reproduced this phenotype. ATRA and retinal, but not retinol increased RAR reporter activity of keratinocytes with impaired RhoA/ROCK signalling, suggesting that retinol metabolism is regulated by RhoA/ROCK signalling. Furthermore a significant percentage of known ATRA target genes displayed altered expression in RhoA-null keratinocytes. These data reveal an unexpected link between the cytoskeletal regulator RhoA and retinoid signalling and uncover a novel pathway by which RhoA regulates gene expression.

AB - The ubiquitously expressed small GTPase RhoA is essential for embryonic development and mutated in different cancers. Functionally, it is well described as a regulator of the actin cytoskeleton, but its role in gene regulation is less understood. Using primary mouse keratinocytes with a deletion of the RhoA gene, we have now been exploring how the loss of RhoA affects gene expression. Performing transcription factor reporter assays, we found a significantly decreased activity of a RAR luciferase reporter in RhoA-null keratinocytes. Inhibition of the RhoA effector ROCK in control cells reproduced this phenotype. ATRA and retinal, but not retinol increased RAR reporter activity of keratinocytes with impaired RhoA/ROCK signalling, suggesting that retinol metabolism is regulated by RhoA/ROCK signalling. Furthermore a significant percentage of known ATRA target genes displayed altered expression in RhoA-null keratinocytes. These data reveal an unexpected link between the cytoskeletal regulator RhoA and retinoid signalling and uncover a novel pathway by which RhoA regulates gene expression.

U2 - 10.1080/21541248.2016.1248272

DO - 10.1080/21541248.2016.1248272

M3 - Journal article

C2 - 27754752

VL - 9

SP - 433

EP - 444

JO - Small GTPases

JF - Small GTPases

SN - 2154-1248

IS - 5

ER -

ID: 167584482