Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets
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Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets. / Linde, S; Hansen, Bruno A.; Welinder, B S; Nielsen, Jens Høiriis.
In: Journal of Chromatography A, Vol. 462, 13.01.1989, p. 243-54.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets
AU - Linde, S
AU - Hansen, Bruno A.
AU - Welinder, B S
AU - Nielsen, Jens Høiriis
PY - 1989/1/13
Y1 - 1989/1/13
N2 - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.
AB - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.
KW - Alleles
KW - Amino Acid Sequence
KW - Animals
KW - C-Peptide
KW - Chromatography, High Pressure Liquid
KW - Insulin
KW - Islets of Langerhans
KW - Mice
KW - Mice, Inbred Strains
KW - Molecular Sequence Data
KW - Proinsulin
KW - Rats
KW - Rats, Inbred Strains
M3 - Journal article
C2 - 2661585
VL - 462
SP - 243
EP - 254
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0301-4770
ER -
ID: 47974424