Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases

Research output: Contribution to journal › Journal article › Research › peer-review

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Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. / Olsen, Lars Folke; Andersen, Ann Zahle; Lunding, Anita; Brasen, Jens Christian; Poulsen, Allan K.

In: Biophysical Journal, Vol. 96, No. 9, 06.05.2009, p. 3850-61.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olsen, LF, Andersen, AZ, Lunding, A, Brasen, JC & Poulsen, AK 2009, 'Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases', Biophysical Journal, vol. 96, no. 9, pp. 3850-61. https://doi.org/10.1016/j.bpj.2009.02.026

APA

Olsen, L. F., Andersen, A. Z., Lunding, A., Brasen, J. C., & Poulsen, A. K. (2009). Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. Biophysical Journal, 96(9), 3850-61. https://doi.org/10.1016/j.bpj.2009.02.026

Vancouver

Olsen LF, Andersen AZ, Lunding A, Brasen JC, Poulsen AK. Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. Biophysical Journal. 2009 May 6;96(9):3850-61. https://doi.org/10.1016/j.bpj.2009.02.026

Author

Olsen, Lars Folke ; Andersen, Ann Zahle ; Lunding, Anita ; Brasen, Jens Christian ; Poulsen, Allan K. / Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. In: Biophysical Journal. 2009 ; Vol. 96, No. 9. pp. 3850-61.

Bibtex

@article{641c86cb59584bf180d5c7f6fd68f548,
title = "Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases",
abstract = "We investigated the coupling between glycolytic and mitochondrial membrane potential oscillations in Saccharomyces cerevisiae under semianaerobic conditions. Glycolysis was measured as NADH autofluorescence, and mitochondrial membrane potential was measured using the fluorescent dye 3,3'-diethyloxacarbocyanine iodide. The responses of glycolytic and membrane potential oscillations to a number of inhibitors of glycolysis, mitochondrial electron flow, and mitochondrial and plasma membrane H(+)-ATPase were investigated. Furthermore, the glycolytic flux was determined as the rate of production of ethanol in a number of different situations (changing pH or the presence and absence of inhibitors). Finally, the intracellular pH was determined and shown to oscillate. The results support earlier work suggesting that the coupling between glycolysis and mitochondrial membrane potential is mediated by the ADP/ATP antiporter and the mitochondrial F(0)F(1)-ATPase. The results further suggest that ATP hydrolysis, through the action of the mitochondrial F(0)F(1)-ATPase and plasma membrane H(+)-ATPase, are important in regulating these oscillations. We conclude that it is glycolysis that drives the oscillations in mitochondrial membrane potential.",
keywords = "Adenosine Triphosphate, Carbon Dioxide, Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone, Cell Membrane, Dithiazanine, Enzyme Inhibitors, Ethanol, Fluorescence, Glycolysis, Hydrogen-Ion Concentration, Membrane Potential, Mitochondrial, Membrane Potentials, Mitochondrial Membranes, NAD, Omeprazole, Oxygen Consumption, Periodicity, Proton-Translocating ATPases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Uncoupling Agents",
author = "Olsen, {Lars Folke} and Andersen, {Ann Zahle} and Anita Lunding and Brasen, {Jens Christian} and Poulsen, {Allan K}",
year = "2009",
month = may,
day = "6",
doi = "10.1016/j.bpj.2009.02.026",
language = "English",
volume = "96",
pages = "3850--61",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases

AU - Olsen, Lars Folke

AU - Andersen, Ann Zahle

AU - Lunding, Anita

AU - Brasen, Jens Christian

AU - Poulsen, Allan K

PY - 2009/5/6

Y1 - 2009/5/6

N2 - We investigated the coupling between glycolytic and mitochondrial membrane potential oscillations in Saccharomyces cerevisiae under semianaerobic conditions. Glycolysis was measured as NADH autofluorescence, and mitochondrial membrane potential was measured using the fluorescent dye 3,3'-diethyloxacarbocyanine iodide. The responses of glycolytic and membrane potential oscillations to a number of inhibitors of glycolysis, mitochondrial electron flow, and mitochondrial and plasma membrane H(+)-ATPase were investigated. Furthermore, the glycolytic flux was determined as the rate of production of ethanol in a number of different situations (changing pH or the presence and absence of inhibitors). Finally, the intracellular pH was determined and shown to oscillate. The results support earlier work suggesting that the coupling between glycolysis and mitochondrial membrane potential is mediated by the ADP/ATP antiporter and the mitochondrial F(0)F(1)-ATPase. The results further suggest that ATP hydrolysis, through the action of the mitochondrial F(0)F(1)-ATPase and plasma membrane H(+)-ATPase, are important in regulating these oscillations. We conclude that it is glycolysis that drives the oscillations in mitochondrial membrane potential.

AB - We investigated the coupling between glycolytic and mitochondrial membrane potential oscillations in Saccharomyces cerevisiae under semianaerobic conditions. Glycolysis was measured as NADH autofluorescence, and mitochondrial membrane potential was measured using the fluorescent dye 3,3'-diethyloxacarbocyanine iodide. The responses of glycolytic and membrane potential oscillations to a number of inhibitors of glycolysis, mitochondrial electron flow, and mitochondrial and plasma membrane H(+)-ATPase were investigated. Furthermore, the glycolytic flux was determined as the rate of production of ethanol in a number of different situations (changing pH or the presence and absence of inhibitors). Finally, the intracellular pH was determined and shown to oscillate. The results support earlier work suggesting that the coupling between glycolysis and mitochondrial membrane potential is mediated by the ADP/ATP antiporter and the mitochondrial F(0)F(1)-ATPase. The results further suggest that ATP hydrolysis, through the action of the mitochondrial F(0)F(1)-ATPase and plasma membrane H(+)-ATPase, are important in regulating these oscillations. We conclude that it is glycolysis that drives the oscillations in mitochondrial membrane potential.

KW - Adenosine Triphosphate

KW - Carbon Dioxide

KW - Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone

KW - Cell Membrane

KW - Dithiazanine

KW - Enzyme Inhibitors

KW - Ethanol

KW - Fluorescence

KW - Glycolysis

KW - Hydrogen-Ion Concentration

KW - Membrane Potential, Mitochondrial

KW - Membrane Potentials

KW - Mitochondrial Membranes

KW - NAD

KW - Omeprazole

KW - Oxygen Consumption

KW - Periodicity

KW - Proton-Translocating ATPases

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Uncoupling Agents

U2 - 10.1016/j.bpj.2009.02.026

DO - 10.1016/j.bpj.2009.02.026

M3 - Journal article

C2 - 19413991

VL - 96

SP - 3850

EP - 3861

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 9

ER -

ID: 33813409