Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice

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Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice. / Schmidtchen, Artur; Puthia, Manoj.

In: Bio-protocol, Vol. 10, No. 16, 3724, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schmidtchen, A & Puthia, M 2020, 'Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice', Bio-protocol, vol. 10, no. 16, 3724. https://doi.org/10.21769/BioProtoc.3724

APA

Schmidtchen, A., & Puthia, M. (2020). Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice. Bio-protocol, 10(16), [3724]. https://doi.org/10.21769/BioProtoc.3724

Vancouver

Schmidtchen A, Puthia M. Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice. Bio-protocol. 2020;10(16). 3724. https://doi.org/10.21769/BioProtoc.3724

Author

Schmidtchen, Artur ; Puthia, Manoj. / Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice. In: Bio-protocol. 2020 ; Vol. 10, No. 16.

Bibtex

@article{cf657c81200149a688dd981874fbd6ad,
title = "Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice",
abstract = "Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on systemic, usually intraperitoneal, administration of lipopolysaccharide (LPS), causing endotoxin shock. Here, we describe a model of LPS-induced local inflammation in NF-kappa B-RE-Luc reporter mice. LPS, alone or with added therapeutic substances, is delivered locally via a hydrogel which is deposited subcutaneously, providing a spatially defined environment, enabling in vivo bioimaging analyses of local NF-kappa B activation. Evaluation of drug efficacy can be analyzed longitudinally in the same mouse, and using fluorescently labeled drugs, local drug deposition can be simultaneously analyzed, and correlated to the site of inflammation. Finally, the protocol can also be used to study retention and systemic release of the drug from locally deposited gels and other biomaterials.",
keywords = "Inflammation, NF-kappa B, In vivo, Mouse model, Bioimaging, Therapy",
author = "Artur Schmidtchen and Manoj Puthia",
year = "2020",
doi = "10.21769/BioProtoc.3724",
language = "English",
volume = "10",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "16",

}

RIS

TY - JOUR

T1 - Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-kappa B Reporter Mice

AU - Schmidtchen, Artur

AU - Puthia, Manoj

PY - 2020

Y1 - 2020

N2 - Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on systemic, usually intraperitoneal, administration of lipopolysaccharide (LPS), causing endotoxin shock. Here, we describe a model of LPS-induced local inflammation in NF-kappa B-RE-Luc reporter mice. LPS, alone or with added therapeutic substances, is delivered locally via a hydrogel which is deposited subcutaneously, providing a spatially defined environment, enabling in vivo bioimaging analyses of local NF-kappa B activation. Evaluation of drug efficacy can be analyzed longitudinally in the same mouse, and using fluorescently labeled drugs, local drug deposition can be simultaneously analyzed, and correlated to the site of inflammation. Finally, the protocol can also be used to study retention and systemic release of the drug from locally deposited gels and other biomaterials.

AB - Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on systemic, usually intraperitoneal, administration of lipopolysaccharide (LPS), causing endotoxin shock. Here, we describe a model of LPS-induced local inflammation in NF-kappa B-RE-Luc reporter mice. LPS, alone or with added therapeutic substances, is delivered locally via a hydrogel which is deposited subcutaneously, providing a spatially defined environment, enabling in vivo bioimaging analyses of local NF-kappa B activation. Evaluation of drug efficacy can be analyzed longitudinally in the same mouse, and using fluorescently labeled drugs, local drug deposition can be simultaneously analyzed, and correlated to the site of inflammation. Finally, the protocol can also be used to study retention and systemic release of the drug from locally deposited gels and other biomaterials.

KW - Inflammation

KW - NF-kappa B

KW - In vivo

KW - Mouse model

KW - Bioimaging

KW - Therapy

U2 - 10.21769/BioProtoc.3724

DO - 10.21769/BioProtoc.3724

M3 - Journal article

C2 - 33659386

VL - 10

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 16

M1 - 3724

ER -

ID: 251695661