Rac1 is crucial for hair follicle integrity but is not essential for maintenance of the epidermis.
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Rac1 is crucial for hair follicle integrity but is not essential for maintenance of the epidermis. / Chrostek, Anna; Wu, Xunwei; Quondamatteo, Fabio; Hu, Rong; Sanecka, Anna; Niemann, Catherin; Langbein, Lutz; Haase, Ingo; Brakebusch, Cord.
In: Molecular and Cellular Biology, Vol. 26, No. 18, 2006, p. 6957-70.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Rac1 is crucial for hair follicle integrity but is not essential for maintenance of the epidermis.
AU - Chrostek, Anna
AU - Wu, Xunwei
AU - Quondamatteo, Fabio
AU - Hu, Rong
AU - Sanecka, Anna
AU - Niemann, Catherin
AU - Langbein, Lutz
AU - Haase, Ingo
AU - Brakebusch, Cord
N1 - Keywords: Animals; Cell Differentiation; Cell Movement; Enzyme Activation; Gene Deletion; Hair Follicle; JNK Mitogen-Activated Protein Kinases; Keratinocytes; Macrophages; Mice; Morphogenesis; NF-kappa B; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-myc; Tamoxifen; p38 Mitogen-Activated Protein Kinases; rac1 GTP-Binding Protein; rho GTP-Binding Proteins
PY - 2006
Y1 - 2006
N2 - Rac1 is a small GTPase that regulates the actin cytoskeleton but also other cellular processes. To investigate the function of Rac1 in skin, we generated mice with a keratinocyte-restricted deletion of the rac1 gene. Rac1-deficient mice lost nearly all of their hair within a few weeks after birth. The nonpermanent part of mutant hair follicles developed constrictions; lost expression of hair follicle-specific keratins, E-cadherin, and alpha6 integrin; and was eventually removed by macrophages. The permanent part of hair follicles and the sebaceous glands were maintained, but no regrowth of full-length hair follicles was observed. In the skin of mutant mice, epidermal keratinocytes showed normal differentiation, proliferation, cell-cell contacts, and basement membrane deposition, demonstrating no obvious defects of Rac1-deficient epidermis in vivo. In vitro, Rac1-null keratinocytes displayed a strong spreading defect and slightly impaired adhesion. These data show that Rac1 plays an important role in sustaining the integrity of the lower part of hair follicles but not in maintenance of the epidermis.
AB - Rac1 is a small GTPase that regulates the actin cytoskeleton but also other cellular processes. To investigate the function of Rac1 in skin, we generated mice with a keratinocyte-restricted deletion of the rac1 gene. Rac1-deficient mice lost nearly all of their hair within a few weeks after birth. The nonpermanent part of mutant hair follicles developed constrictions; lost expression of hair follicle-specific keratins, E-cadherin, and alpha6 integrin; and was eventually removed by macrophages. The permanent part of hair follicles and the sebaceous glands were maintained, but no regrowth of full-length hair follicles was observed. In the skin of mutant mice, epidermal keratinocytes showed normal differentiation, proliferation, cell-cell contacts, and basement membrane deposition, demonstrating no obvious defects of Rac1-deficient epidermis in vivo. In vitro, Rac1-null keratinocytes displayed a strong spreading defect and slightly impaired adhesion. These data show that Rac1 plays an important role in sustaining the integrity of the lower part of hair follicles but not in maintenance of the epidermis.
U2 - 10.1128/MCB.00075-06
DO - 10.1128/MCB.00075-06
M3 - Journal article
C2 - 16943436
VL - 26
SP - 6957
EP - 6970
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 18
ER -
ID: 5140966