TY - JOUR

T1 - Progressive DNA and RNA damage from oxidation after aneurysmal subarachnoid haemorrhage in humans

AU - Jorgensen, Anders

AU - Staalsoe, Jonatan M

AU - Simonsen, Anja H

AU - Hasselbalch, Steen G

AU - Høgh, Peter

AU - Weimann, Allan

AU - Poulsen, Henrik E

AU - Olsen, Neils V

PY - 2018/1

Y1 - 2018/1

N2 - Free radical toxicity is considered as a key mechanism in the neuronal damage occurring after aneurysmal subarachnoid haemorrhage (SAH). We measured markers of DNA and RNA damage from oxidation (8-oxodG and 8-oxoGuo, respectively) in cerebrospinal fluid from 45 patients with SAH on day 1-14 after ictus and 45 age-matched healthy control subjects. At baseline, both markers were significantly increased in patients compared to controls (p values < .001), and exhibited a progressive further increase (to >20-fold above control levels) from day 5-14. None of the markers predicted the occurrence of vasospasms or mortality, although there was a trend that the 8-oxoGuo marker was more strongly associated with mortality than the 8-oxodG marker. We conclude that SAH leads to a massive increase in damage to nucleic acids from oxidative stress, which is likely to play a role in neuronal dysfunction and death. As only patients in need of a ventriculostomy catheter were included in the study, the findings cannot necessarily be extrapolated to all patients with SAH.

AB - Free radical toxicity is considered as a key mechanism in the neuronal damage occurring after aneurysmal subarachnoid haemorrhage (SAH). We measured markers of DNA and RNA damage from oxidation (8-oxodG and 8-oxoGuo, respectively) in cerebrospinal fluid from 45 patients with SAH on day 1-14 after ictus and 45 age-matched healthy control subjects. At baseline, both markers were significantly increased in patients compared to controls (p values < .001), and exhibited a progressive further increase (to >20-fold above control levels) from day 5-14. None of the markers predicted the occurrence of vasospasms or mortality, although there was a trend that the 8-oxoGuo marker was more strongly associated with mortality than the 8-oxodG marker. We conclude that SAH leads to a massive increase in damage to nucleic acids from oxidative stress, which is likely to play a role in neuronal dysfunction and death. As only patients in need of a ventriculostomy catheter were included in the study, the findings cannot necessarily be extrapolated to all patients with SAH.

KW - Journal Article

U2 - 10.1080/10715762.2017.1407413

DO - 10.1080/10715762.2017.1407413

M3 - Journal article

C2 - 29157018

VL - 52

SP - 51

EP - 56

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 1

ER -