Processing of epidermal growth factor in the rat submandibular gland by Kallikrein-like enzymes
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Processing of epidermal growth factor in the rat submandibular gland by Kallikrein-like enzymes. / Jørgensen, P E; Nexø, Ebba; Poulsen, Steen Seier; Almendingen, M; Berg, T.
In: Growth Factors, Vol. 11, No. 2, 1994, p. 113-23.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Processing of epidermal growth factor in the rat submandibular gland by Kallikrein-like enzymes
AU - Jørgensen, P E
AU - Nexø, Ebba
AU - Poulsen, Steen Seier
AU - Almendingen, M
AU - Berg, T
PY - 1994
Y1 - 1994
N2 - Epidermal growth factor (EGF) is synthesized as a precursor which is processed intracellularly to a 6 kDa EGF in the rat submandibular gland. This gland contains very high amounts of kallikrein-like enzymes, and the purpose of the present study was to examine whether any of five such enzymes, rK1, rK2, rK7, rK9 or rK10, can process the rat EGF precursor. Molecular weight forms of EGF, that were N- or C-terminally extended compared to submandibular gland EGF were obtained from rat urine. These extended forms of EGF were incubated with each of the enzymes for 24 h at 37 degrees C. Two enzymes, rK7 and rK10, were able to cleave N- and C-terminally extended EGF, releasing a form of EGF which eluted similarly to submandibular gland EGF upon gel filtration, and which was recognized both by antibodies against rat EGF and by the EGF receptor. One enzyme, rK1, cleaved C- but not N-terminally extended EGF. Neither rK2, nor rK9 cleaved the extended forms of EGF. In previous immunohistochemical studies rK1, rK7 and rK10 have all been demonstrated in the EGF containing cells of the rat submandibular gland. EGF and rK1 are also synthesized in the rat kidney but the present study demonstrated that EGF and rK1 are not colocalized in this organ. Based on the cleavage of the extended forms of rat EGF by rK1, rK7 and rK10 and on the fact that the enzymes are abundant and colocalized with EGF in the rat submandibular gland, we suggest that rK1, rK7 and rK10 can be involved in the processing of the EGF precursor in the rat submandibular gland.
AB - Epidermal growth factor (EGF) is synthesized as a precursor which is processed intracellularly to a 6 kDa EGF in the rat submandibular gland. This gland contains very high amounts of kallikrein-like enzymes, and the purpose of the present study was to examine whether any of five such enzymes, rK1, rK2, rK7, rK9 or rK10, can process the rat EGF precursor. Molecular weight forms of EGF, that were N- or C-terminally extended compared to submandibular gland EGF were obtained from rat urine. These extended forms of EGF were incubated with each of the enzymes for 24 h at 37 degrees C. Two enzymes, rK7 and rK10, were able to cleave N- and C-terminally extended EGF, releasing a form of EGF which eluted similarly to submandibular gland EGF upon gel filtration, and which was recognized both by antibodies against rat EGF and by the EGF receptor. One enzyme, rK1, cleaved C- but not N-terminally extended EGF. Neither rK2, nor rK9 cleaved the extended forms of EGF. In previous immunohistochemical studies rK1, rK7 and rK10 have all been demonstrated in the EGF containing cells of the rat submandibular gland. EGF and rK1 are also synthesized in the rat kidney but the present study demonstrated that EGF and rK1 are not colocalized in this organ. Based on the cleavage of the extended forms of rat EGF by rK1, rK7 and rK10 and on the fact that the enzymes are abundant and colocalized with EGF in the rat submandibular gland, we suggest that rK1, rK7 and rK10 can be involved in the processing of the EGF precursor in the rat submandibular gland.
KW - Animals
KW - Chromatography, Gel
KW - Epidermal Growth Factor
KW - Female
KW - Immunohistochemistry
KW - Kallikreins
KW - Kidney
KW - Male
KW - Rats
KW - Rats, Wistar
KW - Saliva
KW - Submandibular Gland
M3 - Journal article
C2 - 7857656
VL - 11
SP - 113
EP - 123
JO - Growth Factors
JF - Growth Factors
SN - 0897-7194
IS - 2
ER -
ID: 47487269