Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

Research output: Contribution to journal › Journal article › Research › peer-review

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Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae. / Poulsen, Allan K; Andersen, Ann Zahle; Brasen, Jens Christian; Scharff-Poulsen, Anne Marie; Olsen, Lars Folke.

In: Biochemistry, Vol. 47, No. 28, 15.07.2008, p. 7477-84.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Poulsen, AK, Andersen, AZ, Brasen, JC, Scharff-Poulsen, AM & Olsen, LF 2008, 'Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae', Biochemistry, vol. 47, no. 28, pp. 7477-84. https://doi.org/10.1021/bi800396e

APA

Poulsen, A. K., Andersen, A. Z., Brasen, J. C., Scharff-Poulsen, A. M., & Olsen, L. F. (2008). Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae. Biochemistry, 47(28), 7477-84. https://doi.org/10.1021/bi800396e

Vancouver

Poulsen AK, Andersen AZ, Brasen JC, Scharff-Poulsen AM, Olsen LF. Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae. Biochemistry. 2008 Jul 15;47(28):7477-84. https://doi.org/10.1021/bi800396e

Author

Poulsen, Allan K ; Andersen, Ann Zahle ; Brasen, Jens Christian ; Scharff-Poulsen, Anne Marie ; Olsen, Lars Folke. / Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae. In: Biochemistry. 2008 ; Vol. 47, No. 28. pp. 7477-84.

Bibtex

@article{ee667e5e3d3a46ad87515797d50e6b34,
title = "Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae",
abstract = "We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.",
keywords = "Aerobiosis, Anaerobiosis, Biosensing Techniques, Electroporation, Fluorescent Dyes, Glucose, Glycolysis, Kinetics, Membrane Potentials, Nanotechnology, Oscillometry, Oxygen Consumption, Saccharomyces cerevisiae",
author = "Poulsen, {Allan K} and Andersen, {Ann Zahle} and Brasen, {Jens Christian} and Scharff-Poulsen, {Anne Marie} and Olsen, {Lars Folke}",
year = "2008",
month = jul,
day = "15",
doi = "10.1021/bi800396e",
language = "English",
volume = "47",
pages = "7477--84",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "28",

}

RIS

TY - JOUR

T1 - Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

AU - Poulsen, Allan K

AU - Andersen, Ann Zahle

AU - Brasen, Jens Christian

AU - Scharff-Poulsen, Anne Marie

AU - Olsen, Lars Folke

PY - 2008/7/15

Y1 - 2008/7/15

N2 - We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.

AB - We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.

KW - Aerobiosis

KW - Anaerobiosis

KW - Biosensing Techniques

KW - Electroporation

KW - Fluorescent Dyes

KW - Glucose

KW - Glycolysis

KW - Kinetics

KW - Membrane Potentials

KW - Nanotechnology

KW - Oscillometry

KW - Oxygen Consumption

KW - Saccharomyces cerevisiae

U2 - 10.1021/bi800396e

DO - 10.1021/bi800396e

M3 - Journal article

C2 - 18558716

VL - 47

SP - 7477

EP - 7484

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 28

ER -

ID: 33813549