Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability. / Reitelseder, Soren; Tranberg, Britt; Agergaard, Jakob; Dideriksen, Kasper; Hojfeldt, Grith; Merry, Marie Emily; Storm, Adam C.; Poulsen, Kristian R.; Hansen, Erik T.; van Hall, Gerrit; Lund, Peter; Holm, Lars.

In: Clinical Nutrition, Vol. 39, No. 12, 2020, p. 3652-3662.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Reitelseder, S, Tranberg, B, Agergaard, J, Dideriksen, K, Hojfeldt, G, Merry, ME, Storm, AC, Poulsen, KR, Hansen, ET, van Hall, G, Lund, P & Holm, L 2020, 'Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability', Clinical Nutrition, vol. 39, no. 12, pp. 3652-3662. https://doi.org/10.1016/j.clnu.2020.03.017

APA

Reitelseder, S., Tranberg, B., Agergaard, J., Dideriksen, K., Hojfeldt, G., Merry, M. E., Storm, A. C., Poulsen, K. R., Hansen, E. T., van Hall, G., Lund, P., & Holm, L. (2020). Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability. Clinical Nutrition, 39(12), 3652-3662. https://doi.org/10.1016/j.clnu.2020.03.017

Vancouver

Reitelseder S, Tranberg B, Agergaard J, Dideriksen K, Hojfeldt G, Merry ME et al. Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability. Clinical Nutrition. 2020;39(12):3652-3662. https://doi.org/10.1016/j.clnu.2020.03.017

Author

Reitelseder, Soren ; Tranberg, Britt ; Agergaard, Jakob ; Dideriksen, Kasper ; Hojfeldt, Grith ; Merry, Marie Emily ; Storm, Adam C. ; Poulsen, Kristian R. ; Hansen, Erik T. ; van Hall, Gerrit ; Lund, Peter ; Holm, Lars. / Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability. In: Clinical Nutrition. 2020 ; Vol. 39, No. 12. pp. 3652-3662.

Bibtex

@article{bcbfdda9038a404a9dc475b9917f340b,
title = "Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability",
abstract = "Background & aims: Availability of dietary protein-derived amino acids (AA) is an important determinant for their utilization in metabolism and for protein synthesis. Intrinsic labeling of protein is the only method to directly trace availability and utilization. The purpose of the present study was to produce labeled milk and meat proteins and investigate how dietary protein-derived AA availability is affected by the protein-meal matrix.Methods: Four lactating cows were infused with L-[ring-d(5)]phenylalanine and one with L-[N-15]phenylalanine for 72 h. Milk was collected, and three of the [d(5)]phenylalanine cows were subsequently slaughtered. Two human studies were performed to explore plasma AA availability properties utilizing the labeled proteins. One study compared the intake of whey protein either alone or together with carbohydrates-fat food-matrix. The other study compared the intake of meat hydrolysate with minced beef. Cow blood, milk, meat and human blood samples were collected and analyzed by mass spectrometry.Results: Whey and caseinate acquired label to 15-20 mol percent excess (MPE), and the meat proteins reached 0.41-0.73 MPE. The [d(5)]phenylalanine appeared fast in plasma and peaked 30 min after whey protein alone and meat hydrolysate intake, whereas whey protein with a food-matrix and the meat minced beef postponed the [d(5)]phenylalanine peak until 2 and 1 h, respectively.Conclusions: Phenylalanine stable isotope-labeled milk and meat were produced and proved a valuable tool to investigate AA absorption characteristics. Dietary protein in food-matrices showed delayed postprandial plasma AA availability as compared to whey protein alone and meat hydrolysate. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.",
keywords = "Whey, Caseinate, Meat, Protein hydrolysate, Digestion, Amino acid, RESISTANCE EXERCISE, SYNTHESIS RATES, WHEY-PROTEIN, MIXED MEAL, IN-VIVO, OLDER MEN, MUSCLE, INGESTION, METABOLISM, KINETICS",
author = "Soren Reitelseder and Britt Tranberg and Jakob Agergaard and Kasper Dideriksen and Grith Hojfeldt and Merry, {Marie Emily} and Storm, {Adam C.} and Poulsen, {Kristian R.} and Hansen, {Erik T.} and {van Hall}, Gerrit and Peter Lund and Lars Holm",
year = "2020",
doi = "10.1016/j.clnu.2020.03.017",
language = "English",
volume = "39",
pages = "3652--3662",
journal = "Clinical Nutrition",
issn = "0261-5614",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability

AU - Reitelseder, Soren

AU - Tranberg, Britt

AU - Agergaard, Jakob

AU - Dideriksen, Kasper

AU - Hojfeldt, Grith

AU - Merry, Marie Emily

AU - Storm, Adam C.

AU - Poulsen, Kristian R.

AU - Hansen, Erik T.

AU - van Hall, Gerrit

AU - Lund, Peter

AU - Holm, Lars

PY - 2020

Y1 - 2020

N2 - Background & aims: Availability of dietary protein-derived amino acids (AA) is an important determinant for their utilization in metabolism and for protein synthesis. Intrinsic labeling of protein is the only method to directly trace availability and utilization. The purpose of the present study was to produce labeled milk and meat proteins and investigate how dietary protein-derived AA availability is affected by the protein-meal matrix.Methods: Four lactating cows were infused with L-[ring-d(5)]phenylalanine and one with L-[N-15]phenylalanine for 72 h. Milk was collected, and three of the [d(5)]phenylalanine cows were subsequently slaughtered. Two human studies were performed to explore plasma AA availability properties utilizing the labeled proteins. One study compared the intake of whey protein either alone or together with carbohydrates-fat food-matrix. The other study compared the intake of meat hydrolysate with minced beef. Cow blood, milk, meat and human blood samples were collected and analyzed by mass spectrometry.Results: Whey and caseinate acquired label to 15-20 mol percent excess (MPE), and the meat proteins reached 0.41-0.73 MPE. The [d(5)]phenylalanine appeared fast in plasma and peaked 30 min after whey protein alone and meat hydrolysate intake, whereas whey protein with a food-matrix and the meat minced beef postponed the [d(5)]phenylalanine peak until 2 and 1 h, respectively.Conclusions: Phenylalanine stable isotope-labeled milk and meat were produced and proved a valuable tool to investigate AA absorption characteristics. Dietary protein in food-matrices showed delayed postprandial plasma AA availability as compared to whey protein alone and meat hydrolysate. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

AB - Background & aims: Availability of dietary protein-derived amino acids (AA) is an important determinant for their utilization in metabolism and for protein synthesis. Intrinsic labeling of protein is the only method to directly trace availability and utilization. The purpose of the present study was to produce labeled milk and meat proteins and investigate how dietary protein-derived AA availability is affected by the protein-meal matrix.Methods: Four lactating cows were infused with L-[ring-d(5)]phenylalanine and one with L-[N-15]phenylalanine for 72 h. Milk was collected, and three of the [d(5)]phenylalanine cows were subsequently slaughtered. Two human studies were performed to explore plasma AA availability properties utilizing the labeled proteins. One study compared the intake of whey protein either alone or together with carbohydrates-fat food-matrix. The other study compared the intake of meat hydrolysate with minced beef. Cow blood, milk, meat and human blood samples were collected and analyzed by mass spectrometry.Results: Whey and caseinate acquired label to 15-20 mol percent excess (MPE), and the meat proteins reached 0.41-0.73 MPE. The [d(5)]phenylalanine appeared fast in plasma and peaked 30 min after whey protein alone and meat hydrolysate intake, whereas whey protein with a food-matrix and the meat minced beef postponed the [d(5)]phenylalanine peak until 2 and 1 h, respectively.Conclusions: Phenylalanine stable isotope-labeled milk and meat were produced and proved a valuable tool to investigate AA absorption characteristics. Dietary protein in food-matrices showed delayed postprandial plasma AA availability as compared to whey protein alone and meat hydrolysate. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

KW - Whey

KW - Caseinate

KW - Meat

KW - Protein hydrolysate

KW - Digestion

KW - Amino acid

KW - RESISTANCE EXERCISE

KW - SYNTHESIS RATES

KW - WHEY-PROTEIN

KW - MIXED MEAL

KW - IN-VIVO

KW - OLDER MEN

KW - MUSCLE

KW - INGESTION

KW - METABOLISM

KW - KINETICS

U2 - 10.1016/j.clnu.2020.03.017

DO - 10.1016/j.clnu.2020.03.017

M3 - Journal article

C2 - 32334880

VL - 39

SP - 3652

EP - 3662

JO - Clinical Nutrition

JF - Clinical Nutrition

SN - 0261-5614

IS - 12

ER -

ID: 256942978