On-line measurements of oscillating mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae
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On-line measurements of oscillating mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae. / Andersen, Ann Zahle; Poulsen, Allan K; Brasen, Jens Christian; Olsen, Lars Folke; Brasen, Jens Christian.
In: Yeast (Chichester, England), Vol. 24, No. 9, 01.09.2007, p. 731-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - On-line measurements of oscillating mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae
AU - Andersen, Ann Zahle
AU - Poulsen, Allan K
AU - Brasen, Jens Christian
AU - Olsen, Lars Folke
AU - Brasen, Jens Christian
N1 - Copyright (c) 2007 John Wiley & Sons, Ltd.
PY - 2007/9/1
Y1 - 2007/9/1
N2 - We employed the fluorescent cyanine dye DiOC(2)(3) to measure membrane potential in semi-anaerobic yeast cells under conditions where glycolysis was oscillating. Oscillations in glycolysis were studied by means of the naturally abundant nicotinamide adenine dinucleotide (NADH). We found that the mitochondrial membrane potential was oscillating, and that these oscillations displayed the same frequency and duration as the NADH oscillations. It was confirmed that DiOC(2)(3) localizes itself in the mitochondrial membrane and thus reports qualitative changes solely in mitochondrial membrane potential. Our studies showed that glycolytic oscillations perturb the mitochondrial membrane potential and that the mitochondria do not have any controlling effect on the dynamics of glycolysis under these conditions. Depolarization of the mitochondrial membrane by addition of FCCP quenched mitochondrial membrane potential oscillations and delocalized DiOC(2)(3), while glycolysis continued to oscillate unaffected.
AB - We employed the fluorescent cyanine dye DiOC(2)(3) to measure membrane potential in semi-anaerobic yeast cells under conditions where glycolysis was oscillating. Oscillations in glycolysis were studied by means of the naturally abundant nicotinamide adenine dinucleotide (NADH). We found that the mitochondrial membrane potential was oscillating, and that these oscillations displayed the same frequency and duration as the NADH oscillations. It was confirmed that DiOC(2)(3) localizes itself in the mitochondrial membrane and thus reports qualitative changes solely in mitochondrial membrane potential. Our studies showed that glycolytic oscillations perturb the mitochondrial membrane potential and that the mitochondria do not have any controlling effect on the dynamics of glycolysis under these conditions. Depolarization of the mitochondrial membrane by addition of FCCP quenched mitochondrial membrane potential oscillations and delocalized DiOC(2)(3), while glycolysis continued to oscillate unaffected.
KW - Biological Clocks
KW - Carbocyanines
KW - Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
KW - Fluorescent Dyes
KW - Glycolysis
KW - Membrane Potential, Mitochondrial
KW - Microscopy, Fluorescence
KW - Microscopy, Phase-Contrast
KW - NAD
KW - Saccharomyces cerevisiae
KW - Uncoupling Agents
U2 - 10.1002/yea.1508
DO - 10.1002/yea.1508
M3 - Journal article
C2 - 17568453
VL - 24
SP - 731
EP - 739
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 9
ER -
ID: 33803972