Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats

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Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats. / Hey-Mogensen, Martin; Jeppesen, Jacob; Madsen, K; Kiens, Bente; Franch, Jesper.

In: Acta Physiologica (Print), Vol. 204, No. 3, 2012, p. 354-361.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hey-Mogensen, M, Jeppesen, J, Madsen, K, Kiens, B & Franch, J 2012, 'Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats', Acta Physiologica (Print), vol. 204, no. 3, pp. 354-361. https://doi.org/10.1111/j.1748-1716.2011.02347.x

APA

Hey-Mogensen, M., Jeppesen, J., Madsen, K., Kiens, B., & Franch, J. (2012). Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats. Acta Physiologica (Print), 204(3), 354-361. https://doi.org/10.1111/j.1748-1716.2011.02347.x

Vancouver

Hey-Mogensen M, Jeppesen J, Madsen K, Kiens B, Franch J. Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats. Acta Physiologica (Print). 2012;204(3):354-361. https://doi.org/10.1111/j.1748-1716.2011.02347.x

Author

Hey-Mogensen, Martin ; Jeppesen, Jacob ; Madsen, K ; Kiens, Bente ; Franch, Jesper. / Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats. In: Acta Physiologica (Print). 2012 ; Vol. 204, No. 3. pp. 354-361.

Bibtex

@article{3b2e4c0bf92b4a0b9f08bbf5a2706969,
title = "Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats",
abstract = "Aim: Mitochondrial dysfunction has been suggested to play a significant role in obesity and insulin resistance. The aim of the present study was to investigate if changes in obesity and insulin resistance were related to similar changes in mitochondrial capacity for hydrogenperoxide release in Zucker diabetic fatty rats and their lean littermates. Methods: Thirty-four rats were used in this study. Rats were either lean or obese Zucker rats killed at 5-6 (young) or 12-14 (adults) weeks of age. Mitochondria were isolated from soleus muscles; respiration and release of hydrogenperoxide were determined and related to citrate synthase activity to determine intrinsic mitochondrial function. Mitochondrial-specific super-oxide dismuthase (MnSOD) protein content was determined in isolated mitochondria and muscle homogenate. Catalase protein content was determined in muscle homogenate. Results: Young lean and obese rats had a higher mitochondrial respiration when using palmitoyl-l-carnitine as substrate compared with adult lean and obese rats. The obese strain had higher mitochondrial hydrogenperoxide release but only in the adult animals. In both lean and obese animals, increased age was associated with increased mitochondrial hydrogenperoxide release. MnSOD tended to be higher in the obese strain in the isolated mitochondria. Regardless of age, catalase protein content was significantly lower in the obese rats. Conclusions: This study shows that the augmented increase in obesity and insulin resistance seen in Zucker diabetic fatty rats is associated with increased capacity for mitochondrial hydrogenperoxide release.",
author = "Martin Hey-Mogensen and Jacob Jeppesen and K Madsen and Bente Kiens and Jesper Franch",
note = "CURIS 2012 5200 004",
year = "2012",
doi = "10.1111/j.1748-1716.2011.02347.x",
language = "English",
volume = "204",
pages = "354--361",
journal = "Acta Physiologica",
issn = "1748-1708",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Obesity augments the age-induced increase in mitochondrial capacity for H(2) O(2) release in Zucker fatty rats

AU - Hey-Mogensen, Martin

AU - Jeppesen, Jacob

AU - Madsen, K

AU - Kiens, Bente

AU - Franch, Jesper

N1 - CURIS 2012 5200 004

PY - 2012

Y1 - 2012

N2 - Aim: Mitochondrial dysfunction has been suggested to play a significant role in obesity and insulin resistance. The aim of the present study was to investigate if changes in obesity and insulin resistance were related to similar changes in mitochondrial capacity for hydrogenperoxide release in Zucker diabetic fatty rats and their lean littermates. Methods: Thirty-four rats were used in this study. Rats were either lean or obese Zucker rats killed at 5-6 (young) or 12-14 (adults) weeks of age. Mitochondria were isolated from soleus muscles; respiration and release of hydrogenperoxide were determined and related to citrate synthase activity to determine intrinsic mitochondrial function. Mitochondrial-specific super-oxide dismuthase (MnSOD) protein content was determined in isolated mitochondria and muscle homogenate. Catalase protein content was determined in muscle homogenate. Results: Young lean and obese rats had a higher mitochondrial respiration when using palmitoyl-l-carnitine as substrate compared with adult lean and obese rats. The obese strain had higher mitochondrial hydrogenperoxide release but only in the adult animals. In both lean and obese animals, increased age was associated with increased mitochondrial hydrogenperoxide release. MnSOD tended to be higher in the obese strain in the isolated mitochondria. Regardless of age, catalase protein content was significantly lower in the obese rats. Conclusions: This study shows that the augmented increase in obesity and insulin resistance seen in Zucker diabetic fatty rats is associated with increased capacity for mitochondrial hydrogenperoxide release.

AB - Aim: Mitochondrial dysfunction has been suggested to play a significant role in obesity and insulin resistance. The aim of the present study was to investigate if changes in obesity and insulin resistance were related to similar changes in mitochondrial capacity for hydrogenperoxide release in Zucker diabetic fatty rats and their lean littermates. Methods: Thirty-four rats were used in this study. Rats were either lean or obese Zucker rats killed at 5-6 (young) or 12-14 (adults) weeks of age. Mitochondria were isolated from soleus muscles; respiration and release of hydrogenperoxide were determined and related to citrate synthase activity to determine intrinsic mitochondrial function. Mitochondrial-specific super-oxide dismuthase (MnSOD) protein content was determined in isolated mitochondria and muscle homogenate. Catalase protein content was determined in muscle homogenate. Results: Young lean and obese rats had a higher mitochondrial respiration when using palmitoyl-l-carnitine as substrate compared with adult lean and obese rats. The obese strain had higher mitochondrial hydrogenperoxide release but only in the adult animals. In both lean and obese animals, increased age was associated with increased mitochondrial hydrogenperoxide release. MnSOD tended to be higher in the obese strain in the isolated mitochondria. Regardless of age, catalase protein content was significantly lower in the obese rats. Conclusions: This study shows that the augmented increase in obesity and insulin resistance seen in Zucker diabetic fatty rats is associated with increased capacity for mitochondrial hydrogenperoxide release.

U2 - 10.1111/j.1748-1716.2011.02347.x

DO - 10.1111/j.1748-1716.2011.02347.x

M3 - Journal article

C2 - 21827638

VL - 204

SP - 354

EP - 361

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 3

ER -

ID: 34420955