NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs
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NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs. / Bertram, Hanne Christine S.; Malmendal, Anders; Nielsen, Niels Chr; Straadt, Ida Krestine; Larsen, Torben; Knudsen, Knud Erik Bach; Laerke, Helle Nygaard.
In: Molecular Nutrition & Food Research (Online), Vol. 53, No. 8, 2009, p. 1055-62.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs
AU - Bertram, Hanne Christine S.
AU - Malmendal, Anders
AU - Nielsen, Niels Chr
AU - Straadt, Ida Krestine
AU - Larsen, Torben
AU - Knudsen, Knud Erik Bach
AU - Laerke, Helle Nygaard
PY - 2009
Y1 - 2009
N2 - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.
AB - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.
KW - Animals
KW - Betaine
KW - Cholesterol
KW - Female
KW - Magnetic Resonance Spectroscopy
KW - Metabolomics
KW - Secale cereale
KW - Swine
U2 - 10.1002/mnfr.200800344
DO - 10.1002/mnfr.200800344
M3 - Journal article
C2 - 19603403
VL - 53
SP - 1055
EP - 1062
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 8
ER -
ID: 33167015