Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity
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Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. / Kraunsøe, Regitze; Boushel, Robert Christopher; Hansen, Christina Neigaard; Schjerling, Peter; Qvortrup, Klaus; Støckel, Mikael; Mikines, Kári J; Dela, Flemming.
In: Journal of Physiology, Vol. 588, No. 12, 2010, p. 2023-2032.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity
AU - Kraunsøe, Regitze
AU - Boushel, Robert Christopher
AU - Hansen, Christina Neigaard
AU - Schjerling, Peter
AU - Qvortrup, Klaus
AU - Støckel, Mikael
AU - Mikines, Kári J
AU - Dela, Flemming
PY - 2010
Y1 - 2010
N2 - Adipose tissue exerts important endocrine and metabolic functions in health and disease. Yet the bioenergetics of this tissue is not characterized in humans and possible regional differences are not elucidated. Using high resolution respirometry, mitochondrial respiration was quantified in human abdominal subcutaneous and intra-abdominal visceral (omentum majus) adipose tissue from biopsies obtained in 20 obese patients undergoing bariatric surgery. Mitochondrial DNA (mtDNA) and genomic DNA (gDNA) were determined by the PCR technique for estimation of mitochondrial density. Adipose tissue samples were permeabilized and respirometric measurements were performed in duplicate at 37 degrees C. Substrates (glutamate (G) + malate (M) + octanoyl carnitine (O) + succinate (S)) were added sequentially to provide electrons to complex I + II. ADP ((D)) for state 3 respiration was added after GM. Uncoupled respiration was measured after addition of FCCP. Visceral fat contained more mitochondria per milligram of tissue than subcutaneous fat, but the cells were smaller. Robust, stable oxygen fluxes were found in both tissues, and coupled state 3 (GMOS(D)) and uncoupled respiration were significantly (P <0.05) higher in visceral (0.95 +/- 0.05 and 1.15 +/- 0.06 pmol O(2) s(1) mg(1), respectively) compared with subcutaneous (0.76 +/- 0.04 and 0.98 +/- 0.05 pmol O(2) s(1) mg(1), respectively) adipose tissue. Expressed per mtDNA, visceral adipose tissue had significantly (P <0.05) lower mitochondrial respiration. Substrate control ratios were higher and uncoupling control ratio lower (P <0.05) in visceral compared with subcutaneous adipose tissue. We conclude that visceral fat is bioenergetically more active and more sensitive to mitochondrial substrate supply than subcutaneous fat. Oxidative phosphorylation has a higher relative activity in visceral compared with subcutaneous adipose tissue.
AB - Adipose tissue exerts important endocrine and metabolic functions in health and disease. Yet the bioenergetics of this tissue is not characterized in humans and possible regional differences are not elucidated. Using high resolution respirometry, mitochondrial respiration was quantified in human abdominal subcutaneous and intra-abdominal visceral (omentum majus) adipose tissue from biopsies obtained in 20 obese patients undergoing bariatric surgery. Mitochondrial DNA (mtDNA) and genomic DNA (gDNA) were determined by the PCR technique for estimation of mitochondrial density. Adipose tissue samples were permeabilized and respirometric measurements were performed in duplicate at 37 degrees C. Substrates (glutamate (G) + malate (M) + octanoyl carnitine (O) + succinate (S)) were added sequentially to provide electrons to complex I + II. ADP ((D)) for state 3 respiration was added after GM. Uncoupled respiration was measured after addition of FCCP. Visceral fat contained more mitochondria per milligram of tissue than subcutaneous fat, but the cells were smaller. Robust, stable oxygen fluxes were found in both tissues, and coupled state 3 (GMOS(D)) and uncoupled respiration were significantly (P <0.05) higher in visceral (0.95 +/- 0.05 and 1.15 +/- 0.06 pmol O(2) s(1) mg(1), respectively) compared with subcutaneous (0.76 +/- 0.04 and 0.98 +/- 0.05 pmol O(2) s(1) mg(1), respectively) adipose tissue. Expressed per mtDNA, visceral adipose tissue had significantly (P <0.05) lower mitochondrial respiration. Substrate control ratios were higher and uncoupling control ratio lower (P <0.05) in visceral compared with subcutaneous adipose tissue. We conclude that visceral fat is bioenergetically more active and more sensitive to mitochondrial substrate supply than subcutaneous fat. Oxidative phosphorylation has a higher relative activity in visceral compared with subcutaneous adipose tissue.
KW - Adult
KW - Biopsy
KW - Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
KW - Carnitine
KW - Cell Respiration
KW - DNA, Mitochondrial
KW - Energy Metabolism
KW - Female
KW - Glutamic Acid
KW - Humans
KW - Intra-Abdominal Fat
KW - Malates
KW - Male
KW - Microscopy, Electron, Transmission
KW - Mitochondria
KW - Obesity, Morbid
KW - Omentum
KW - Oxidative Phosphorylation
KW - Subcutaneous Fat, Abdominal
KW - Succinic Acid
KW - Time Factors
KW - Uncoupling Agents
U2 - 10.1113/jphysiol.2009.184754
DO - 10.1113/jphysiol.2009.184754
M3 - Journal article
C2 - 20421291
VL - 588
SP - 2023
EP - 2032
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 12
ER -
ID: 33815847