MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice. / Fang, Shu; Feng, Jie; Zhang, Hongbin; Li, Ping; Zhang, Yudan; Zeng, Yanmei; Cai, Yingying; Lin, Xiaochun; Xue, Yaoming; Guan, Meiping.

In: Adipocyte, Vol. 9, No. 1, 2020, p. 179-188.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fang, S, Feng, J, Zhang, H, Li, P, Zhang, Y, Zeng, Y, Cai, Y, Lin, X, Xue, Y & Guan, M 2020, 'MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice', Adipocyte, vol. 9, no. 1, pp. 179-188. https://doi.org/10.1080/21623945.2020.1749495

APA

Fang, S., Feng, J., Zhang, H., Li, P., Zhang, Y., Zeng, Y., Cai, Y., Lin, X., Xue, Y., & Guan, M. (2020). MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice. Adipocyte, 9(1), 179-188. https://doi.org/10.1080/21623945.2020.1749495

Vancouver

Fang S, Feng J, Zhang H, Li P, Zhang Y, Zeng Y et al. MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice. Adipocyte. 2020;9(1):179-188. https://doi.org/10.1080/21623945.2020.1749495

Author

Fang, Shu ; Feng, Jie ; Zhang, Hongbin ; Li, Ping ; Zhang, Yudan ; Zeng, Yanmei ; Cai, Yingying ; Lin, Xiaochun ; Xue, Yaoming ; Guan, Meiping. / MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice. In: Adipocyte. 2020 ; Vol. 9, No. 1. pp. 179-188.

Bibtex

@article{6a364390ff1e4669a5e0fb12dae5d8c8,
title = "MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice",
abstract = "MiR-455 has been verified a key regulator of brown adipose tissue and adipose tissue-specific overexpression of miR-455 (ap2-miR-455) mice could combat high-fat-diet-induced obesity. This study is to verify overexpression of miR-455 could ameliorate the lipid accumulation and metabolism in the liver of db/db diabetic mice and explore the potential mechanisms. Diabetic mice (db/db) and control mice (db/m) were randomly divided into four groups. After overexpression of miR-455 in the liver of db/db mice, the triglycerides level in both serum and liver decreased, the lipid deposit in liver was improved, the expression of fatty acid synthase, stearoyl-CoA desaturase 1, sterol regulatory element binding protein 1c (SREBP-1c) and acetyl-CoA carboxylase (ACCα) was also significantly down-regulated. TargetScan indicated that suppressor of cytokine signalling 3 (SOCS3) is predicated to target miR-455 and the protein of SOCS3 in the liver of db/db mice after intervention was significantly decreased. The dual luciferase reporter assay showed that SOCS3 was target gene of miR-455. In vitro, in Palmitate (PA)-stimulated human normal liver (LO2) cells, transfected miR-455 mimic could significantly inhibit the expression of SOCS3, while transfected miR-455 inhibitor could up-regulate the expression of SOCS3. Transfecting LO2 cells with siRNA of SOCS3 could significantly down-regulate the protein expression of SREBP-1c and ACCα. Our study showed that overexpression of miR-455 in the liver could improve lipid metabolism in diabetic mice by down-regulating its target gene SOCS3.",
keywords = "Diabetes, lipid metabolism, miR-455, NAFLD, SOCS3",
author = "Shu Fang and Jie Feng and Hongbin Zhang and Ping Li and Yudan Zhang and Yanmei Zeng and Yingying Cai and Xiaochun Lin and Yaoming Xue and Meiping Guan",
year = "2020",
doi = "10.1080/21623945.2020.1749495",
language = "English",
volume = "9",
pages = "179--188",
journal = "Adipocytes",
issn = "1550-2082",
publisher = "New Century Health Publishers, LLC",
number = "1",

}

RIS

TY - JOUR

T1 - MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice

AU - Fang, Shu

AU - Feng, Jie

AU - Zhang, Hongbin

AU - Li, Ping

AU - Zhang, Yudan

AU - Zeng, Yanmei

AU - Cai, Yingying

AU - Lin, Xiaochun

AU - Xue, Yaoming

AU - Guan, Meiping

PY - 2020

Y1 - 2020

N2 - MiR-455 has been verified a key regulator of brown adipose tissue and adipose tissue-specific overexpression of miR-455 (ap2-miR-455) mice could combat high-fat-diet-induced obesity. This study is to verify overexpression of miR-455 could ameliorate the lipid accumulation and metabolism in the liver of db/db diabetic mice and explore the potential mechanisms. Diabetic mice (db/db) and control mice (db/m) were randomly divided into four groups. After overexpression of miR-455 in the liver of db/db mice, the triglycerides level in both serum and liver decreased, the lipid deposit in liver was improved, the expression of fatty acid synthase, stearoyl-CoA desaturase 1, sterol regulatory element binding protein 1c (SREBP-1c) and acetyl-CoA carboxylase (ACCα) was also significantly down-regulated. TargetScan indicated that suppressor of cytokine signalling 3 (SOCS3) is predicated to target miR-455 and the protein of SOCS3 in the liver of db/db mice after intervention was significantly decreased. The dual luciferase reporter assay showed that SOCS3 was target gene of miR-455. In vitro, in Palmitate (PA)-stimulated human normal liver (LO2) cells, transfected miR-455 mimic could significantly inhibit the expression of SOCS3, while transfected miR-455 inhibitor could up-regulate the expression of SOCS3. Transfecting LO2 cells with siRNA of SOCS3 could significantly down-regulate the protein expression of SREBP-1c and ACCα. Our study showed that overexpression of miR-455 in the liver could improve lipid metabolism in diabetic mice by down-regulating its target gene SOCS3.

AB - MiR-455 has been verified a key regulator of brown adipose tissue and adipose tissue-specific overexpression of miR-455 (ap2-miR-455) mice could combat high-fat-diet-induced obesity. This study is to verify overexpression of miR-455 could ameliorate the lipid accumulation and metabolism in the liver of db/db diabetic mice and explore the potential mechanisms. Diabetic mice (db/db) and control mice (db/m) were randomly divided into four groups. After overexpression of miR-455 in the liver of db/db mice, the triglycerides level in both serum and liver decreased, the lipid deposit in liver was improved, the expression of fatty acid synthase, stearoyl-CoA desaturase 1, sterol regulatory element binding protein 1c (SREBP-1c) and acetyl-CoA carboxylase (ACCα) was also significantly down-regulated. TargetScan indicated that suppressor of cytokine signalling 3 (SOCS3) is predicated to target miR-455 and the protein of SOCS3 in the liver of db/db mice after intervention was significantly decreased. The dual luciferase reporter assay showed that SOCS3 was target gene of miR-455. In vitro, in Palmitate (PA)-stimulated human normal liver (LO2) cells, transfected miR-455 mimic could significantly inhibit the expression of SOCS3, while transfected miR-455 inhibitor could up-regulate the expression of SOCS3. Transfecting LO2 cells with siRNA of SOCS3 could significantly down-regulate the protein expression of SREBP-1c and ACCα. Our study showed that overexpression of miR-455 in the liver could improve lipid metabolism in diabetic mice by down-regulating its target gene SOCS3.

KW - Diabetes

KW - lipid metabolism

KW - miR-455

KW - NAFLD

KW - SOCS3

UR - http://www.scopus.com/inward/record.url?scp=85083498201&partnerID=8YFLogxK

U2 - 10.1080/21623945.2020.1749495

DO - 10.1080/21623945.2020.1749495

M3 - Journal article

C2 - 32272865

AN - SCOPUS:85083498201

VL - 9

SP - 179

EP - 188

JO - Adipocytes

JF - Adipocytes

SN - 1550-2082

IS - 1

ER -

ID: 247037894