Matters of fiber size and myonuclear domain: does size matter more than age?

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Matters of fiber size and myonuclear domain : does size matter more than age? / Karlsen, Anders; Couppé, Christian; Andersen, Jesper L.; Mikkelsen, Ulla R.; Nielsen, Rie H.; Magnusson, Stig Peter; Kjaer, Michael; Mackey, Abigail.

In: Muscle & Nerve, Vol. 52, No. 6, 12.2015, p. 1040-1046.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Karlsen, A, Couppé, C, Andersen, JL, Mikkelsen, UR, Nielsen, RH, Magnusson, SP, Kjaer, M & Mackey, A 2015, 'Matters of fiber size and myonuclear domain: does size matter more than age?', Muscle & Nerve, vol. 52, no. 6, pp. 1040-1046. https://doi.org/10.1002/mus.24669

APA

Karlsen, A., Couppé, C., Andersen, J. L., Mikkelsen, U. R., Nielsen, R. H., Magnusson, S. P., Kjaer, M., & Mackey, A. (2015). Matters of fiber size and myonuclear domain: does size matter more than age? Muscle & Nerve, 52(6), 1040-1046. https://doi.org/10.1002/mus.24669

Vancouver

Karlsen A, Couppé C, Andersen JL, Mikkelsen UR, Nielsen RH, Magnusson SP et al. Matters of fiber size and myonuclear domain: does size matter more than age? Muscle & Nerve. 2015 Dec;52(6):1040-1046. https://doi.org/10.1002/mus.24669

Author

Karlsen, Anders ; Couppé, Christian ; Andersen, Jesper L. ; Mikkelsen, Ulla R. ; Nielsen, Rie H. ; Magnusson, Stig Peter ; Kjaer, Michael ; Mackey, Abigail. / Matters of fiber size and myonuclear domain : does size matter more than age?. In: Muscle & Nerve. 2015 ; Vol. 52, No. 6. pp. 1040-1046.

Bibtex

@article{272f84da4f0c4459ae1dfe209f88bf8f,
title = "Matters of fiber size and myonuclear domain: does size matter more than age?",
abstract = "INTRODUCTION: The relationship between fiber size and myonuclear content is understood poorly.METHODS: Biopsy cross-sections from young and old trained and untrained healthy individuals were analyzed for fiber area and myonuclei, and 2 fiber size-dependent cluster analyses were performed.RESULTS: Comparing fibers of similar size, no effect of training or age was found for myonuclear domain. There was a linear relationship between fiber area and myonuclei per fiber (r=0.99; P<0.001) and a non-linear relationship between fiber area and domain (r=0.97-0.99; P<0.0001), with a markedly smaller domain in fibers <3,000 μm(2) . A larger proportion of type II fibers below 3,000 μm(2) was observed in the old.DISCUSSION: These findings suggest that age-related reductions in myonuclear domain size could be explained by a larger proportion of small fibers and highlight the usefulness of fiber size-based clusters in gaining mechanistic insight into the relationship between skeletal muscle fiber size and myonuclear content.",
author = "Anders Karlsen and Christian Coupp{\'e} and Andersen, {Jesper L.} and Mikkelsen, {Ulla R.} and Nielsen, {Rie H.} and Magnusson, {Stig Peter} and Michael Kjaer and Abigail Mackey",
note = "{\textcopyright} 2015 Wiley Periodicals, Inc.",
year = "2015",
month = dec,
doi = "10.1002/mus.24669",
language = "English",
volume = "52",
pages = "1040--1046",
journal = "Muscle & Nerve",
issn = "0148-639X",
publisher = "JohnWiley & Sons, Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Matters of fiber size and myonuclear domain

T2 - does size matter more than age?

AU - Karlsen, Anders

AU - Couppé, Christian

AU - Andersen, Jesper L.

AU - Mikkelsen, Ulla R.

AU - Nielsen, Rie H.

AU - Magnusson, Stig Peter

AU - Kjaer, Michael

AU - Mackey, Abigail

N1 - © 2015 Wiley Periodicals, Inc.

PY - 2015/12

Y1 - 2015/12

N2 - INTRODUCTION: The relationship between fiber size and myonuclear content is understood poorly.METHODS: Biopsy cross-sections from young and old trained and untrained healthy individuals were analyzed for fiber area and myonuclei, and 2 fiber size-dependent cluster analyses were performed.RESULTS: Comparing fibers of similar size, no effect of training or age was found for myonuclear domain. There was a linear relationship between fiber area and myonuclei per fiber (r=0.99; P<0.001) and a non-linear relationship between fiber area and domain (r=0.97-0.99; P<0.0001), with a markedly smaller domain in fibers <3,000 μm(2) . A larger proportion of type II fibers below 3,000 μm(2) was observed in the old.DISCUSSION: These findings suggest that age-related reductions in myonuclear domain size could be explained by a larger proportion of small fibers and highlight the usefulness of fiber size-based clusters in gaining mechanistic insight into the relationship between skeletal muscle fiber size and myonuclear content.

AB - INTRODUCTION: The relationship between fiber size and myonuclear content is understood poorly.METHODS: Biopsy cross-sections from young and old trained and untrained healthy individuals were analyzed for fiber area and myonuclei, and 2 fiber size-dependent cluster analyses were performed.RESULTS: Comparing fibers of similar size, no effect of training or age was found for myonuclear domain. There was a linear relationship between fiber area and myonuclei per fiber (r=0.99; P<0.001) and a non-linear relationship between fiber area and domain (r=0.97-0.99; P<0.0001), with a markedly smaller domain in fibers <3,000 μm(2) . A larger proportion of type II fibers below 3,000 μm(2) was observed in the old.DISCUSSION: These findings suggest that age-related reductions in myonuclear domain size could be explained by a larger proportion of small fibers and highlight the usefulness of fiber size-based clusters in gaining mechanistic insight into the relationship between skeletal muscle fiber size and myonuclear content.

U2 - 10.1002/mus.24669

DO - 10.1002/mus.24669

M3 - Journal article

C2 - 25845994

VL - 52

SP - 1040

EP - 1046

JO - Muscle & Nerve

JF - Muscle & Nerve

SN - 0148-639X

IS - 6

ER -

ID: 135274229