Matrix-insensitive protein assays push the limits of biosensors in medicine
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Matrix-insensitive protein assays push the limits of biosensors in medicine. / Gaster, Richard S; Hall, Drew A; Nielsen, Carsten Haagen; Osterfeld, Sebastian J; Yu, Heng; Mach, Kathleen E; Wilson, Robert J; Murmann, Boris; Liao, Joseph C; Gambhir, Sanjiv S; Wang, Shan X.
In: Nature Medicine, Vol. 15, No. 11, 2009, p. 1327-1332.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Matrix-insensitive protein assays push the limits of biosensors in medicine
AU - Gaster, Richard S
AU - Hall, Drew A
AU - Nielsen, Carsten Haagen
AU - Osterfeld, Sebastian J
AU - Yu, Heng
AU - Mach, Kathleen E
AU - Wilson, Robert J
AU - Murmann, Boris
AU - Liao, Joseph C
AU - Gambhir, Sanjiv S
AU - Wang, Shan X
N1 - Keywords: Animals; Biological Assay; Biosensing Techniques; Carcinoembryonic Antigen; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Mice; Nanotechnology; Optics and Photonics; Proteins; Reproducibility of Results; Sensitivity and Specificity; Temperature; Time Factors; Vascular Endothelial Growth Factor A; Xenograft Model Antitumor Assays
PY - 2009
Y1 - 2009
N2 - Advances in biosensor technologies for in vitro diagnostics have the potential to transform the practice of medicine. Despite considerable work in the biosensor field, there is still no general sensing platform that can be ubiquitously applied to detect the constellation of biomolecules in diverse clinical samples (for example, serum, urine, cell lysates or saliva) with high sensitivity and large linear dynamic range. A major limitation confounding other technologies is signal distortion that occurs in various matrices due to heterogeneity in ionic strength, pH, temperature and autofluorescence. Here we present a magnetic nanosensor technology that is matrix insensitive yet still capable of rapid, multiplex protein detection with resolution down to attomolar concentrations and extensive linear dynamic range. The matrix insensitivity of our platform to various media demonstrates that our magnetic nanosensor technology can be directly applied to a variety of settings such as molecular biology, clinical diagnostics and biodefense.
AB - Advances in biosensor technologies for in vitro diagnostics have the potential to transform the practice of medicine. Despite considerable work in the biosensor field, there is still no general sensing platform that can be ubiquitously applied to detect the constellation of biomolecules in diverse clinical samples (for example, serum, urine, cell lysates or saliva) with high sensitivity and large linear dynamic range. A major limitation confounding other technologies is signal distortion that occurs in various matrices due to heterogeneity in ionic strength, pH, temperature and autofluorescence. Here we present a magnetic nanosensor technology that is matrix insensitive yet still capable of rapid, multiplex protein detection with resolution down to attomolar concentrations and extensive linear dynamic range. The matrix insensitivity of our platform to various media demonstrates that our magnetic nanosensor technology can be directly applied to a variety of settings such as molecular biology, clinical diagnostics and biodefense.
U2 - 10.1038/nm.2032
DO - 10.1038/nm.2032
M3 - Journal article
C2 - 19820717
VL - 15
SP - 1327
EP - 1332
JO - Nature Medicine
JF - Nature Medicine
SN - 1078-8956
IS - 11
ER -
ID: 20195002