Loss of high-molecular-weight cytokeratin antigenicity in prostate tissue obtained by transurethral resections.
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Loss of high-molecular-weight cytokeratin antigenicity in prostate tissue obtained by transurethral resections. / Multhaupt, H A; Fessler, J N; Warhol, M J.
In: Archives of Pathology & Laboratory Medicine, Vol. 124, No. 12, 2000, p. 1764-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Loss of high-molecular-weight cytokeratin antigenicity in prostate tissue obtained by transurethral resections.
AU - Multhaupt, H A
AU - Fessler, J N
AU - Warhol, M J
N1 - Keywords: Adenocarcinoma; Antibodies, Monoclonal; Biopsy, Needle; Diagnosis, Differential; Humans; Immunohistochemistry; Keratins; Male; Molecular Weight; Prostate; Prostatectomy; Prostatic Neoplasms; Transurethral Resection of Prostate
PY - 2000
Y1 - 2000
N2 - OBJECTIVE: Staining of prostatic basal cells for the expression of high-molecular-weight cytokeratin has been suggested as a way of distinguishing benign from malignant prostate glands. We evaluated the utility of high-molecular-weight cytokeratin in the diagnosis of malignancy in prostate specimens obtained in various ways. DESIGN: Prostate tissues obtained from needle biopsies, transurethral resections, and total prostatectomies were immunostained with monoclonal antibody 34betaE12, an antibody directed against high-molecular-weight cytokeratins. RESULTS: Antiserum to high-molecular-weight cytokeratin only stained the basal cells in normal glands in 3 (12%) of 25 specimens obtained by transurethral resection. Other antigens, such as the alternate 10-nm filament protein vimentin, were unaffected and were detected in 100% of these specimens. However, keratin antigenicity in transurethral biopsies could be restored in these specimens by antigen retrieval in a low pH citrate buffer using a microwave heat technique. Keratin staining in needle biopsies and total prostatectomies was unaffected. CONCLUSION: In summary, our results indicate the technique of transurethral resection results in a specific loss of keratin antigenicity. This limits the utility of anticytokeratin 34betaE12 in interpreting transurethral resections without the application of antigen retrieval.
AB - OBJECTIVE: Staining of prostatic basal cells for the expression of high-molecular-weight cytokeratin has been suggested as a way of distinguishing benign from malignant prostate glands. We evaluated the utility of high-molecular-weight cytokeratin in the diagnosis of malignancy in prostate specimens obtained in various ways. DESIGN: Prostate tissues obtained from needle biopsies, transurethral resections, and total prostatectomies were immunostained with monoclonal antibody 34betaE12, an antibody directed against high-molecular-weight cytokeratins. RESULTS: Antiserum to high-molecular-weight cytokeratin only stained the basal cells in normal glands in 3 (12%) of 25 specimens obtained by transurethral resection. Other antigens, such as the alternate 10-nm filament protein vimentin, were unaffected and were detected in 100% of these specimens. However, keratin antigenicity in transurethral biopsies could be restored in these specimens by antigen retrieval in a low pH citrate buffer using a microwave heat technique. Keratin staining in needle biopsies and total prostatectomies was unaffected. CONCLUSION: In summary, our results indicate the technique of transurethral resection results in a specific loss of keratin antigenicity. This limits the utility of anticytokeratin 34betaE12 in interpreting transurethral resections without the application of antigen retrieval.
M3 - Journal article
C2 - 11100054
VL - 124
SP - 1764
EP - 1767
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
SN - 0003-9985
IS - 12
ER -
ID: 5240250