Intermittent Dynamic Compression Confers Anabolic Effects in Articular Cartilage
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Intermittent Dynamic Compression Confers Anabolic Effects in Articular Cartilage. / Engstrom, Amalie; Gillesberg, Frederik S.; Groen, Solveig S.; Frederiksen, Peder; Bay-Jensen, Anne-Christine; Karsdal, Morten A.; Thudium, Christian S.
In: Applied Sciences, Vol. 11, No. 16, 7469, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Intermittent Dynamic Compression Confers Anabolic Effects in Articular Cartilage
AU - Engstrom, Amalie
AU - Gillesberg, Frederik S.
AU - Groen, Solveig S.
AU - Frederiksen, Peder
AU - Bay-Jensen, Anne-Christine
AU - Karsdal, Morten A.
AU - Thudium, Christian S.
PY - 2021
Y1 - 2021
N2 - Featured Application This work shows the use of an ex vivo cartilage compression model to assess the effect of combining compressive loading and growth factors on cartilage remodeling. (1) Background: Mechanical loading is an essential part of the function and maintenance of the joint. Despite the importance of intermittent mechanical loading, this factor is rarely considered in preclinical models of cartilage, limiting their translatability. The aim of this study was to investigate the effects of intermittent dynamic compression on the extracellular matrix during long-term culture of bovine cartilage explants. (2) Methods: Bovine articular cartilage explants were cultured for 21 days and subjected to 20 min of 1 Hz cyclic compressive loading five consecutive days each week. Cartilage remodeling was investigated in the presence of IGF-1 or TGF-beta 1, as well as a TGF-beta receptor 1 (ALK5) kinase inhibitor and assessed with biomarkers for type II collagen formation (PRO-C2) and fibronectin degradation (FBN-C). (3) Results: Compression of cartilage explants increased the release of PRO-C2 and FBN-C to the conditioned media and, furthermore, IGF-1 and compression synergistically increased PRO-C2 release. Inhibition of ALK5 blocked PRO-C2 and FBN-C release in dynamically compressed explants. (4) Conclusions: Dynamic compression of cartilage explants increases both type II collagen formation and fibronectin degradation, and IGF-1 interacts synergistically with compression, increasing the overall impact on cartilage formation. These data show that mechanical loading is important to consider in translational cartilage models.
AB - Featured Application This work shows the use of an ex vivo cartilage compression model to assess the effect of combining compressive loading and growth factors on cartilage remodeling. (1) Background: Mechanical loading is an essential part of the function and maintenance of the joint. Despite the importance of intermittent mechanical loading, this factor is rarely considered in preclinical models of cartilage, limiting their translatability. The aim of this study was to investigate the effects of intermittent dynamic compression on the extracellular matrix during long-term culture of bovine cartilage explants. (2) Methods: Bovine articular cartilage explants were cultured for 21 days and subjected to 20 min of 1 Hz cyclic compressive loading five consecutive days each week. Cartilage remodeling was investigated in the presence of IGF-1 or TGF-beta 1, as well as a TGF-beta receptor 1 (ALK5) kinase inhibitor and assessed with biomarkers for type II collagen formation (PRO-C2) and fibronectin degradation (FBN-C). (3) Results: Compression of cartilage explants increased the release of PRO-C2 and FBN-C to the conditioned media and, furthermore, IGF-1 and compression synergistically increased PRO-C2 release. Inhibition of ALK5 blocked PRO-C2 and FBN-C release in dynamically compressed explants. (4) Conclusions: Dynamic compression of cartilage explants increases both type II collagen formation and fibronectin degradation, and IGF-1 interacts synergistically with compression, increasing the overall impact on cartilage formation. These data show that mechanical loading is important to consider in translational cartilage models.
KW - osteoarthritis
KW - articular cartilage
KW - dynamic compression
KW - bovine explants
KW - type II collagen
KW - fibronectin
KW - translational research
KW - GROWTH-FACTOR-I
KW - FACTOR-BETA
KW - PROTEOGLYCAN SYNTHESIS
KW - EXTRACELLULAR-MATRIX
KW - HYDROSTATIC-PRESSURE
KW - HYPERTROPHIC DIFFERENTIATION
KW - MECHANICAL COMPRESSION
KW - GENE-EXPRESSION
KW - KNEE CARTILAGE
KW - TGF-BETA
U2 - 10.3390/app11167469
DO - 10.3390/app11167469
M3 - Journal article
VL - 11
JO - Applied Sciences
JF - Applied Sciences
SN - 1454-5101
IS - 16
M1 - 7469
ER -
ID: 286918611