Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma

Research output: Contribution to journal › Journal article › Research › peer-review

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Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma. / Wu, Chunyan; Zhang, Huijian; Zhang, Jiajun; Zhang, Hongbin; Zeng, Yanmei; Fang, Shu; Li, Ping; Zhang, Yudan; Lin, Xiaochun; Wang, Ling; Xue, Yaoming; Guan, Meiping.

In: Adipocyte, Vol. 8, No. 1, 2019, p. 347-356.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wu, C, Zhang, H, Zhang, J, Zhang, H, Zeng, Y, Fang, S, Li, P, Zhang, Y, Lin, X, Wang, L, Xue, Y & Guan, M 2019, 'Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma', Adipocyte, vol. 8, no. 1, pp. 347-356. https://doi.org/10.1080/21623945.2019.1690834

APA

Wu, C., Zhang, H., Zhang, J., Zhang, H., Zeng, Y., Fang, S., Li, P., Zhang, Y., Lin, X., Wang, L., Xue, Y., & Guan, M. (2019). Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma. Adipocyte, 8(1), 347-356. https://doi.org/10.1080/21623945.2019.1690834

Vancouver

Wu C, Zhang H, Zhang J, Zhang H, Zeng Y, Fang S et al. Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma. Adipocyte. 2019;8(1):347-356. https://doi.org/10.1080/21623945.2019.1690834

Author

Wu, Chunyan ; Zhang, Huijian ; Zhang, Jiajun ; Zhang, Hongbin ; Zeng, Yanmei ; Fang, Shu ; Li, Ping ; Zhang, Yudan ; Lin, Xiaochun ; Wang, Ling ; Xue, Yaoming ; Guan, Meiping. / Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma. In: Adipocyte. 2019 ; Vol. 8, No. 1. pp. 347-356.

Bibtex

@article{95c0e617023e4f4396632e78f9ab1e1e,
title = "Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma",
abstract = "Although much is known about that corticosteroids affect the functions of adipose tissues, little genetic information is available for perirenal adipose tissue (peri-N) from patients with cortisol-producing adenoma (CPA). We conducted microarray analysis of peri-N from patients with CPA by using an Affymetrix human U133 plus 2.0 array. We also analysed the inflammation, fibrosis and oxidative stress in vitro. Compared with normotension (NT) group, CPA group has significantly higher protein levels of TNF alpha, IL-6, fibronectin (FN) and collagen I (COLI). The protein level of NADPH oxidase 4 (Nox4) significantly increased, while nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) levels were significantly reduced in the CPA group. Dexamethasone markedly induced fibrosis and adipogenesis-related gene expression in predifferentiated stromal vascular fraction (SVF) cells, 3T3-L1 preadipocytes and brown preadipocytes. Chronic exposure to endogenous glucocorticoids due to CPA increases peri-N oxidative stress, inflammation and fibrosis, which may contribute to the metabolic disturbances associated with hypercortisolism in these patients.",
keywords = "Cushing's syndrome, oxidative stress, inflammation, fibrosis, adipose tissue, microarray",
author = "Chunyan Wu and Huijian Zhang and Jiajun Zhang and Hongbin Zhang and Yanmei Zeng and Shu Fang and Ping Li and Yudan Zhang and Xiaochun Lin and Ling Wang and Yaoming Xue and Meiping Guan",
year = "2019",
doi = "10.1080/21623945.2019.1690834",
language = "English",
volume = "8",
pages = "347--356",
journal = "Adipocytes",
issn = "1550-2082",
publisher = "New Century Health Publishers, LLC",
number = "1",

}

RIS

TY - JOUR

T1 - Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma

AU - Wu, Chunyan

AU - Zhang, Huijian

AU - Zhang, Jiajun

AU - Zhang, Hongbin

AU - Zeng, Yanmei

AU - Fang, Shu

AU - Li, Ping

AU - Zhang, Yudan

AU - Lin, Xiaochun

AU - Wang, Ling

AU - Xue, Yaoming

AU - Guan, Meiping

PY - 2019

Y1 - 2019

N2 - Although much is known about that corticosteroids affect the functions of adipose tissues, little genetic information is available for perirenal adipose tissue (peri-N) from patients with cortisol-producing adenoma (CPA). We conducted microarray analysis of peri-N from patients with CPA by using an Affymetrix human U133 plus 2.0 array. We also analysed the inflammation, fibrosis and oxidative stress in vitro. Compared with normotension (NT) group, CPA group has significantly higher protein levels of TNF alpha, IL-6, fibronectin (FN) and collagen I (COLI). The protein level of NADPH oxidase 4 (Nox4) significantly increased, while nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) levels were significantly reduced in the CPA group. Dexamethasone markedly induced fibrosis and adipogenesis-related gene expression in predifferentiated stromal vascular fraction (SVF) cells, 3T3-L1 preadipocytes and brown preadipocytes. Chronic exposure to endogenous glucocorticoids due to CPA increases peri-N oxidative stress, inflammation and fibrosis, which may contribute to the metabolic disturbances associated with hypercortisolism in these patients.

AB - Although much is known about that corticosteroids affect the functions of adipose tissues, little genetic information is available for perirenal adipose tissue (peri-N) from patients with cortisol-producing adenoma (CPA). We conducted microarray analysis of peri-N from patients with CPA by using an Affymetrix human U133 plus 2.0 array. We also analysed the inflammation, fibrosis and oxidative stress in vitro. Compared with normotension (NT) group, CPA group has significantly higher protein levels of TNF alpha, IL-6, fibronectin (FN) and collagen I (COLI). The protein level of NADPH oxidase 4 (Nox4) significantly increased, while nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) levels were significantly reduced in the CPA group. Dexamethasone markedly induced fibrosis and adipogenesis-related gene expression in predifferentiated stromal vascular fraction (SVF) cells, 3T3-L1 preadipocytes and brown preadipocytes. Chronic exposure to endogenous glucocorticoids due to CPA increases peri-N oxidative stress, inflammation and fibrosis, which may contribute to the metabolic disturbances associated with hypercortisolism in these patients.

KW - Cushing's syndrome

KW - oxidative stress

KW - inflammation

KW - fibrosis

KW - adipose tissue

KW - microarray

U2 - 10.1080/21623945.2019.1690834

DO - 10.1080/21623945.2019.1690834

M3 - Journal article

C2 - 31718404

VL - 8

SP - 347

EP - 356

JO - Adipocytes

JF - Adipocytes

SN - 1550-2082

IS - 1

ER -

ID: 230691887