In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts
Research output: Contribution to journal › Journal article › Research › peer-review
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Original language | English |
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Journal | Nature Methods |
Volume | 6 |
Issue number | 2 |
Pages (from-to) | 143-5 |
Number of pages | 2 |
ISSN | 1548-7091 |
DOIs | |
Publication status | Published - 2009 |
Bibliographical note
Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells
ID: 18764243