Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism. / Olsen, Hervør L; Nørby, Peder L; Høy, Marianne; Spee, Pieter; Thams, Peter; Capito, Kirsten; Petersen, Jacob S; Gromada, Jesper.

In: Biochemical and Biophysical Research Communications, Vol. 303, No. 4, 2003, p. 1148-51.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olsen, HL, Nørby, PL, Høy, M, Spee, P, Thams, P, Capito, K, Petersen, JS & Gromada, J 2003, 'Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism.', Biochemical and Biophysical Research Communications, vol. 303, no. 4, pp. 1148-51.

APA

Olsen, H. L., Nørby, P. L., Høy, M., Spee, P., Thams, P., Capito, K., Petersen, J. S., & Gromada, J. (2003). Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism. Biochemical and Biophysical Research Communications, 303(4), 1148-51.

Vancouver

Olsen HL, Nørby PL, Høy M, Spee P, Thams P, Capito K et al. Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism. Biochemical and Biophysical Research Communications. 2003;303(4):1148-51.

Author

Olsen, Hervør L ; Nørby, Peder L ; Høy, Marianne ; Spee, Pieter ; Thams, Peter ; Capito, Kirsten ; Petersen, Jacob S ; Gromada, Jesper. / Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 303, No. 4. pp. 1148-51.

Bibtex

@article{79c2c040af2511ddb538000ea68e967b,
title = "Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism.",
abstract = "We have previously demonstrated that the novel imidazoline compound (+)-2-(2-(4,5-dihydro-1H-imidazol-2-yl)-thiopene-2-yl-ethyl)-pyridine (NNC77-0074) increases insulin secretion from pancreatic beta-cells by stimulation of Ca(2+)-dependent exocytosis. Using capacitance measurements, we now show that NNC77-0074 stimulates exocytosis in clonal INS-1E cells. NNC77-0074-stimulated exocytosis was antagonised by the cytoplasmic phospholipase A(2) (cPLA(2)) inhibitors ACA and AACOCF(3) and in cells treated with antisense oligonucleotide against cPLA(2)alpha. NNC77-0074-evoked insulin secretion was likewise inhibited by ACA, AACOCF(3), and cPLA(2)alpha antisense oligonucleotide treatment. In pancreatic islets NNC77-0074 stimulated PLA(2) activity. We propose that cPLA(2)alpha plays an important role in the regulation of NNC77-0074-evoked exocytosis in insulin secreting beta-cells.",
author = "Olsen, {Herv{\o}r L} and N{\o}rby, {Peder L} and Marianne H{\o}y and Pieter Spee and Peter Thams and Kirsten Capito and Petersen, {Jacob S} and Jesper Gromada",
note = "Keywords: Animals; Calcium Signaling; Cells, Cultured; Enzyme Inhibitors; Exocytosis; Group IV Phospholipases A2; Imidazoles; Islets of Langerhans; Male; Mice; Oligonucleotides, Antisense; Phospholipases A; Phospholipases A2; Pyridines; Tumor Cells, Cultured",
year = "2003",
language = "English",
volume = "303",
pages = "1148--51",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Imidazoline NNC77-0074 stimulates Ca2+-evoked exocytosis in INS-1E cells by a phospholipase A2-dependent mechanism.

AU - Olsen, Hervør L

AU - Nørby, Peder L

AU - Høy, Marianne

AU - Spee, Pieter

AU - Thams, Peter

AU - Capito, Kirsten

AU - Petersen, Jacob S

AU - Gromada, Jesper

N1 - Keywords: Animals; Calcium Signaling; Cells, Cultured; Enzyme Inhibitors; Exocytosis; Group IV Phospholipases A2; Imidazoles; Islets of Langerhans; Male; Mice; Oligonucleotides, Antisense; Phospholipases A; Phospholipases A2; Pyridines; Tumor Cells, Cultured

PY - 2003

Y1 - 2003

N2 - We have previously demonstrated that the novel imidazoline compound (+)-2-(2-(4,5-dihydro-1H-imidazol-2-yl)-thiopene-2-yl-ethyl)-pyridine (NNC77-0074) increases insulin secretion from pancreatic beta-cells by stimulation of Ca(2+)-dependent exocytosis. Using capacitance measurements, we now show that NNC77-0074 stimulates exocytosis in clonal INS-1E cells. NNC77-0074-stimulated exocytosis was antagonised by the cytoplasmic phospholipase A(2) (cPLA(2)) inhibitors ACA and AACOCF(3) and in cells treated with antisense oligonucleotide against cPLA(2)alpha. NNC77-0074-evoked insulin secretion was likewise inhibited by ACA, AACOCF(3), and cPLA(2)alpha antisense oligonucleotide treatment. In pancreatic islets NNC77-0074 stimulated PLA(2) activity. We propose that cPLA(2)alpha plays an important role in the regulation of NNC77-0074-evoked exocytosis in insulin secreting beta-cells.

AB - We have previously demonstrated that the novel imidazoline compound (+)-2-(2-(4,5-dihydro-1H-imidazol-2-yl)-thiopene-2-yl-ethyl)-pyridine (NNC77-0074) increases insulin secretion from pancreatic beta-cells by stimulation of Ca(2+)-dependent exocytosis. Using capacitance measurements, we now show that NNC77-0074 stimulates exocytosis in clonal INS-1E cells. NNC77-0074-stimulated exocytosis was antagonised by the cytoplasmic phospholipase A(2) (cPLA(2)) inhibitors ACA and AACOCF(3) and in cells treated with antisense oligonucleotide against cPLA(2)alpha. NNC77-0074-evoked insulin secretion was likewise inhibited by ACA, AACOCF(3), and cPLA(2)alpha antisense oligonucleotide treatment. In pancreatic islets NNC77-0074 stimulated PLA(2) activity. We propose that cPLA(2)alpha plays an important role in the regulation of NNC77-0074-evoked exocytosis in insulin secreting beta-cells.

M3 - Journal article

C2 - 12684056

VL - 303

SP - 1148

EP - 1151

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

ID: 8522104