Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons
Research output: Contribution to journal › Journal article › Research › peer-review
A preparative purification of endogenous inhibitors of the Na+/K+-ATPase has been carried out from bovine blood. Dried plasma was deproteinized, hexane-extracted and desalted, followed by further purification through a series of reverse-phase HPLC fractionations. Fractions active in inhibiting Na+/K+-ATPase activity and displacing ouabain were collected and purified further. By comparison with ouabain, the final extract was found to have a steeper concentration-effect curve in the inhibition of Na+/K+-ATPase. In displacement of [3H]ouabain, the extract had again a steeper concentration-effect curve than does ouabain, and in addition it enhanced ouabain binding at high dilutions. These properties are indicative of nonspecific interactions with the Na+/K+-ATPase. The active fraction was identified by TLC, HPLC, NMR, GLC and GC-MS, to be a mixture of three unesterified fatty acids, mainly oleic acid (72% of the total) and three saturated hydrocarbons. The assignment of structures was corroborated by comparison with authentic samples.
Original language | English |
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Journal | BBA General Subjects |
Volume | 985 |
Issue number | 1 |
Pages (from-to) | 55-9 |
Number of pages | 4 |
ISSN | 0304-4165 |
Publication status | Published - 1989 |
Bibliographical note
Keywords: Animals; Cattle; Chromatography, Gas; Chromatography, High Pressure Liquid; Enzyme Inhibitors; Fatty Acids; Gas Chromatography-Mass Spectrometry; Hydrocarbons; Kidney Medulla; Ouabain; Sodium-Potassium-Exchanging ATPase; Swine
ID: 12485088