Identification of a KCNE2 gain-of-function mutation in patients with familial atrial fibrillation
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Identification of a KCNE2 gain-of-function mutation in patients with familial atrial fibrillation. / Yang, Yiqing; Xia, Min; Jin, Qingfeng; Bendahhou, Saïd; Shi, Jingyi; Chen, Yiping; Liang, Bo; Lin, Jie; Liu, Yi; Liu, Ban; Zhou, Qinshu; Zhang, Dongwei; Wang, Rong; Ma, Ning; Su, Xiaoyan; Niu, Kaiya; Pei, Yan; Xu, Wenyuan; Chen, Zhaopeng; Wan, Haiying; Cui, Jianmin; Barhanin, Jacques; Chen, Yihan.
In: American Journal of Human Genetics, Vol. 75, No. 5, 2004, p. 899-905.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of a KCNE2 gain-of-function mutation in patients with familial atrial fibrillation
AU - Yang, Yiqing
AU - Xia, Min
AU - Jin, Qingfeng
AU - Bendahhou, Saïd
AU - Shi, Jingyi
AU - Chen, Yiping
AU - Liang, Bo
AU - Lin, Jie
AU - Liu, Yi
AU - Liu, Ban
AU - Zhou, Qinshu
AU - Zhang, Dongwei
AU - Wang, Rong
AU - Ma, Ning
AU - Su, Xiaoyan
AU - Niu, Kaiya
AU - Pei, Yan
AU - Xu, Wenyuan
AU - Chen, Zhaopeng
AU - Wan, Haiying
AU - Cui, Jianmin
AU - Barhanin, Jacques
AU - Chen, Yihan
PY - 2004
Y1 - 2004
N2 - Atrial fibrillation (AF) is the most common cardiac arrhythmia encountered in clinical practice. We first reported an S140G mutation of KCNQ1, an alpha subunit of potassium channels, in one Chinese kindred with AF. However, the molecular defects and cellular mechanisms in most patients with AF remain to be identified. We evaluated 28 unrelated Chinese kindreds with AF and sequenced eight genes of potassium channels (KCNQ1, HERG, KCNE1, KCNE2, KCNE3, KCNE4, KCNE5, and KCNJ2). An arginine-to-cysteine mutation at position 27 (R27C) of KCNE2, the beta subunit of the KCNQ1-KCNE2 channel responsible for a background potassium current, was found in 2 of the 28 probands. The mutation was present in all affected members in the two kindreds and was absent in 462 healthy unrelated Chinese subjects. Similar to KCNQ1 S140G, the mutation had a gain-of-function effect on the KCNQ1-KCNE2 channel; unlike long QT syndrome-associated KCNE2 mutations, it did not alter HERG-KCNE2 current. The mutation did not alter the functions of the HCN channel family either. Thus, KCNE2 R27C is a gain-of-function mutation associated with the initiation and/or maintenance of AF.
AB - Atrial fibrillation (AF) is the most common cardiac arrhythmia encountered in clinical practice. We first reported an S140G mutation of KCNQ1, an alpha subunit of potassium channels, in one Chinese kindred with AF. However, the molecular defects and cellular mechanisms in most patients with AF remain to be identified. We evaluated 28 unrelated Chinese kindreds with AF and sequenced eight genes of potassium channels (KCNQ1, HERG, KCNE1, KCNE2, KCNE3, KCNE4, KCNE5, and KCNJ2). An arginine-to-cysteine mutation at position 27 (R27C) of KCNE2, the beta subunit of the KCNQ1-KCNE2 channel responsible for a background potassium current, was found in 2 of the 28 probands. The mutation was present in all affected members in the two kindreds and was absent in 462 healthy unrelated Chinese subjects. Similar to KCNQ1 S140G, the mutation had a gain-of-function effect on the KCNQ1-KCNE2 channel; unlike long QT syndrome-associated KCNE2 mutations, it did not alter HERG-KCNE2 current. The mutation did not alter the functions of the HCN channel family either. Thus, KCNE2 R27C is a gain-of-function mutation associated with the initiation and/or maintenance of AF.
KW - Animals
KW - Atrial Fibrillation
KW - Base Sequence
KW - COS Cells
KW - Cercopithecus aethiops
KW - China
KW - Electrocardiography
KW - Female
KW - Humans
KW - Ion Transport
KW - Male
KW - Middle Aged
KW - Molecular Sequence Data
KW - Mutation, Missense
KW - Pedigree
KW - Potassium
KW - Potassium Channels
KW - Potassium Channels, Voltage-Gated
KW - Sequence Analysis, DNA
KW - Transfection
U2 - 10.1086/425342
DO - 10.1086/425342
M3 - Journal article
C2 - 15368194
VL - 75
SP - 899
EP - 905
JO - American Journal of Human Genetics
JF - American Journal of Human Genetics
SN - 0002-9297
IS - 5
ER -
ID: 40249271